ArticleThe Review of scientific instruments2003
Optical workstation with concurrent, independent multiphoton imaging and experimental laser microbeam capabilities.
Article in The Review of scientific instruments, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- A novel bioreactor for combined magnetic resonance spectroscopy and optical imaging of metabolism in 3D cell cultures.Magnetic resonance in medicine · 2019Article
- Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine.Journal of visualized experiments : JoVE · 2019Article
- Autofluorescence lifetime imaging of cellular metabolism: Sensitivity toward cell density, pH, intracellular, and intercellular heterogeneity.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Radiation Promptly Alters Cancer Live Cell Metabolic Fluxes: An In Vitro Demonstration.Radiation research · 2016Article
- RhoA is down-regulated at cell-cell contacts via p190RhoGAP-B in response to tensional homeostasis.Molecular biology of the cell · 2013Article
- Nonlinear optical microscopy and ultrasound imaging of human cervical structure.Journal of biomedical optics · 2013Article
- Quantification of collagen organization and extracellular matrix factors within the healing ligament.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2011Article
- Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging.Nature protocols · 2011Article
- Multiphoton flow cytometry to assess intrinsic and extrinsic fluorescence in cellular aggregates: applications to stem cells.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2011Article
- Integrated holographic system for all-optical manipulation of developing embryos.Biomedical optics express · 2011Article
- Visualization of morphological and molecular features associated with chronic ischemia in bioengineered human skin.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2010Article
- CGEF-1 and CHIN-1 regulate CDC-42 activity during asymmetric division in the Caenorhabditis elegans embryo.Molecular biology of the cell · 2010Article
- Multiphoton redox ratio imaging for metabolic monitoring in vivo.Methods in molecular biology (Clifton, N.J.) · 2010Article
- Shining new light on 3D cell motility and the metastatic process.Trends in cell biology · 2009Review
- Control of 3-dimensional collagen matrix polymerization for reproducible human mammary fibroblast cell culture in microfluidic devices.Biomaterials · 2009Article
- Filamin A-beta1 integrin complex tunes epithelial cell response to matrix tension.Molecular biology of the cell · 2009Article
- Dynamin participates in the maintenance of anterior polarity in the Caenorhabditis elegans embryo.Developmental cell · 2009Article
- Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancer.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2009Article
- Nonlinear optical imaging of cellular processes in breast cancer.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2008Article
- RAB-11 permissively regulates spindle alignment by modulating metaphase microtubule dynamics in Caenorhabditis elegans early embryos.Molecular biology of the cell · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Experimental laser microbeam techniques have become established tools for studying living specimens. A steerable, focused laser beam may be used for a variety of experimental manipulations such as laser microsurgery, optical trapping, localized photolysis of caged bioactive probes, and patterned photobleaching. Typically, purpose-designed experimental systems have been constructed for each of these applications. In order to assess the consequences of such experimental optical interventions, long-term, microscopic observation of the specimen is often required. Multiphoton excitation, because of its ability to obtain high-contrast images from deep within a specimen with minimal phototoxic effects, is a preferred technique for in vivo imaging. An optical workstation is described that combines the functionality of an experimental optical microbeam apparatus with a sensitive multiphoton imaging system designed for use with living specimens. Design considerations are discussed and examples of ongoing biological applications are presented. The integrated optical workstation concept offers advantages in terms of flexibility and versatility relative to systems implemented with separate imaging and experimental components.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.