ArticleJournal of bacteriology2008
Toxic introns and parasitic intein in Coxiella burnetii: legacies of a promiscuous past.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Inteins as Drug Targets and Therapeutic Tools.Frontiers in molecular biosciences · 2022Review
- A CsrA-Binding,Journal of bacteriology · 2019Article
- Identification of novel MITEs (miniature inverted-repeat transposable elements) in Coxiella burnetii: implications for protein and small RNA evolution.BMC genomics · 2018Article
- Horizontally Acquired Biosynthesis Genes BoostFrontiers in cellular and infection microbiology · 2017Article
- Impact of a homing intein on recombination frequency and organismal fitness.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Intervening Sequence of Coxiella burnetii: Characterization and Evolution.Frontiers in cellular and infection microbiology · 2016Article
- Article
- Identification of novel small RNAs and characterization of the 6S RNA of Coxiella burnetii.PloS one · 2014Article
- Article
- Genetics of Coxiella burnetii: on the path of specialization.Future microbiology · 2011Review
- Ribozyme stability, exon skipping, and a potential role for RNA helicase in group I intron splicing by Coxiella burnetii.Journal of bacteriology · 2011Article
- A unique Coxiella burnetii lipoprotein involved in metal binding (LimB).Microbiology (Reading, England) · 2011Article
- Cisplatin inhibits protein splicing, suggesting inteins as therapeutic targets in mycobacteria.The Journal of biological chemistry · 2011Article
- Advances in genetic manipulation of obligate intracellular bacterial pathogens.Frontiers in microbiology · 2011Article
- A DNA-binding peroxiredoxin of Coxiella burnetii is involved in countering oxidative stress during exponential-phase growth.Journal of bacteriology · 2010Article
- Group I introns and inteins: disparate origins but convergent parasitic strategies.Journal of bacteriology · 2009Review
- A unique group I intron in Coxiella burnetii is a natural splice mutant.Journal of bacteriology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The genome of the obligate intracellular pathogen Coxiella burnetii contains a large number of selfish genetic elements, including two group I introns (Cbu.L1917 and Cbu.L1951) and an intervening sequence that interrupts the 23S rRNA gene, an intein (Cbu.DnaB) within dnaB and 29 insertion sequences. Here, we describe the ability of the intron-encoded RNAs (ribozymes) to retard bacterial growth rate (toxicity) and examine the functionality and phylogenetic history of Cbu.DnaB. When expressed in Escherichia coli, both introns repressed growth, with Cbu.L1917 being more inhibitory. Both ribozymes were found to associate with ribosomes of Coxiella and E. coli. In addition, ribozymes significantly reduced in vitro luciferase translation, again with Cbu.L1917 being more inhibitory. We analyzed the relative quantities of ribozymes and genomes throughout a 14-day growth cycle of C. burnetii and found that they were inversely correlated, suggesting that the ribozymes have a negative effect on Coxiella's growth. We determined possible sites for ribozyme associations with 23S rRNA that could explain the observed toxicities. Further research is needed to determine whether the introns are being positively selected because they promote bacterial persistence or whether they were fixed in the population due to genetic drift. The intein, Cbu.DnaB, is able to self-splice, leaving the host protein intact and presumably functional. Similar inteins have been found in two extremophilic bacteria (Alkalilimnicola ehrlichei and Halorhodospira halophila) that are distantly related to Coxiella, making it difficult to determine whether the intein was acquired by horizontal gene transfer or was vertically inherited from a common ancestor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.