ArticleMicrobial pathogenesis2008
Outer membrane protein A expression in Enterobacter sakazakii is required to induce microtubule condensation in human brain microvascular endothelial cells for invasion.
Article in Microbial pathogenesis, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 76 citations in OpenAlex.
- DecipheringMicroorganisms · 2026Review
- Pathogenic characterization and mechanism of sequence type 4 Cronobacter sakazakii derived from milk-based infant and baby foods.BMC microbiology · 2025Article
- Functional Characterization of a Novel SMR-Type Efflux Pump RanQ, Mediating Quaternary Ammonium Compound Resistance inMicroorganisms · 2023Article
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- Emergence ofFrontiers in microbiology · 2020Article
- The OmpA Gene of Xanthomonas axonopodis pv. glycines is Involved in Pathogenesis of Pustule Disease on Soybean.Current microbiology · 2019Article
- Genomic Analysis of Putative Virulence Factors Affecting Cytotoxicity ofFrontiers in microbiology · 2019Article
- Expression ofFood science and biotechnology · 2018Article
- Virulence and Antibiotic Resistance Profiles ofFrontiers in microbiology · 2018Article
- Article
- Prevalence, Molecular Characterization, and Antibiotic Susceptibility ofFrontiers in microbiology · 2017Article
- Analysis and Characterization of Proteins Associated with Outer Membrane Vesicles Secreted byFrontiers in microbiology · 2017Article
- Genotyping and Source Tracking of Cronobacter sakazakii and C. malonaticus Isolates from Powdered Infant Formula and an Infant Formula Production Factory in China.Applied and environmental microbiology · 2015Article
- Infectious causes of necrotizing enterocolitis.Clinics in perinatology · 2015Review
- Plasmid-encoded MCP is involved in virulence, motility, and biofilm formation of Cronobacter sakazakii ATCC 29544.Infection and immunity · 2015Article
- Insights into virulence factors determining the pathogenicity of Cronobacter sakazakii.Virulence · 2015Review
- Sub-inhibitory concentrations of trans-cinnamaldehyde attenuate virulence in Cronobacter sakazakii in vitro.International journal of molecular sciences · 2014Article
- Cronobacter: an emerging opportunistic pathogen associated with neonatal meningitis, sepsis and necrotizing enterocolitis.Journal of perinatology : official journal of the California Perinatal Association · 2013Review
- Complete genome sequence of the opportunistic food-borne pathogen Cronobacter sakazakii ES15.Journal of bacteriology · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Enterobacter sakazakii (ES) causes neonatal meningitis and necrotizing enterocolitis with case-fatality rates among infected infants ranging from 40 to 80%. Very little is known about the mechanisms by which these organisms cause disease. Here, we demonstrate that ES invades human brain microvascular endothelial cells (HBMEC) with higher frequency when compared with epithelial cells and endothelial cells from different origins. The entry of ES into HBMEC requires the expression of outer membrane protein A (OmpA), as the OmpA-deletion mutant was sevenfold less invasive than the wild type ES and the bacterium does not multiply inside HBMEC. Anti-OmpA antibodies generated against the OmpA of Escherichia coli K1, which also recognize the OmpA of ES, did not prevent the invasion of ES in HBMEC. ES invasion depends on microtubule condensation in HBMEC and is independent of actin filament reorganization. Both PI3-kinase and PKC-alpha were activated during ES entry into HBMEC between 15 min and 30 min of infection. Concomitantly, overexpression of dominant negative forms of PI3-kinase and PKC-alpha significantly inhibited the invasion of ES into HBMEC. In summary, ES invasion of HBMEC is dependent on the expression of OmpA similar to that of E. coli K1; however, the epitopes involved in the interaction with HBMEC appears to be different.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.