ArticleMolecular immunology2008
The 'zinc knuckle' motif of Early B cell Factor is required for transcriptional activation of B cell-specific genes.
Article in Molecular immunology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Fine mapping of a major QTL, qECQ8, for rice taste quality.BMC plant biology · 2024Article
- Zinc finger proteins: insights into the transcriptional and post transcriptional regulation of immune response.Molecular biology reports · 2021Review
- Early B Cell Factor Activity Controls Developmental and Adaptive Thermogenic Gene Programming in Adipocytes.Cell reports · 2020Article
- The structure, splicing, synteny and expression of lamprey COE genes and the evolution of the COE gene family in chordates.Development genes and evolution · 2017Article
- Novel de novo variant inCold Spring Harbor molecular case studies · 2017Article
- Mutations in EBF3 Disturb Transcriptional Profiles and Cause Intellectual Disability, Ataxia, and Facial Dysmorphism.American journal of human genetics · 2017Article
- De Novo Mutations in EBF3 Cause a Neurodevelopmental Syndrome.American journal of human genetics · 2017Article
- What maintains the high intra-follicular estradiol concentration in pre-ovulatory follicles?Journal of assisted reproduction and genetics · 2016Article
- Early B-cell factor 3 (EBF3) is a novel tumor suppressor gene with promoter hypermethylation in pediatric acute myeloid leukemia.Journal of experimental & clinical cancer research : CR · 2015Article
- ZNF423 and ZNF521: EBF1 Antagonists of Potential Relevance in B-Lymphoid Malignancies.BioMed research international · 2015Review
- The effect of RA on the chick Ebf1-3 genes expression in somites and pharyngeal arches.Development genes and evolution · 2014Article
- The regulatory network of B-cell differentiation: a focused view of early B-cell factor 1 function.Immunological reviews · 2014Review
- Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Activation of Aicda gene transcription by Pax5 in plasmacytoma cells.Immunologic research · 2013Article
- B lymphocyte lineage specification, commitment and epigenetic control of transcription by early B cell factor 1.Current topics in microbiology and immunology · 2012Review
- Structural determination of functional domains in early B-cell factor (EBF) family of transcription factors reveals similarities to Rel DNA-binding proteins and a novel dimerization motif.The Journal of biological chemistry · 2010Article
- Compound haploinsufficiencies of Ebf1 and Runx1 genes impede B cell lineage progression.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Emerging roles of the EBF family of transcription factors in tumor suppression.Molecular cancer research : MCR · 2009Review
- Opposing effects of SWI/SNF and Mi-2/NuRD chromatin remodeling complexes on epigenetic reprogramming by EBF and Pax5.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Ebf1-mediated down-regulation of Id2 and Id3 is essential for specification of the B cell lineage.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Early B cell factor (EBF) is a critical regulator of B lymphocyte-specific gene transcription. EBF functions, in part, by binding to regulatory sites of genes required for the pre-B- and mature B cell receptors. These DNA targets include the promoters of the mb-1 and Vpreb1 genes that encode Ig-alpha and one of the components of surrogate light chain, respectively. The biochemical basis of DNA binding and gene activation by EBF is poorly understood. The DNA-binding domain (DBD) of EBF includes a putative zinc-binding motif (HX(3)CX(2)CX(5)C), which we have designated the 'Zn-knuckle'. The Zn-knuckle is required for binding of the mb-1 promoter site in EMSA, but it has not been demonstrated to be important for functional activities of EBF in B cells. Therefore, we expressed EBF with mutations in the Zn-knuckle motif or flanking sequences in plasmacytoma cells in which activation of endogenous mb-1 and Vpreb1 genes is dependent on EBF. EBF with mutations that prevent zinc coordination by the Zn-knuckle did not activate transcription of either target gene. Other mutations affected the sequence preference of DNA binding and differentially inhibited activation of these genes. Our results demonstrate the importance of the Zn-knuckle motif in EBF. These experiments also confirm that EBF can re-activate multiple genes of the early B cell program in plasmacytoma cells, which provide a useful cell-based assay for dissecting mechanisms involving EBF.
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