ArticleBMC genomics2008
The ORFeome of Staphylococcus aureus v 1.1.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox for use in vertebrate model systems.Development (Cambridge, England) · 2025Article
- Generating genomic platforms to study Candida albicans pathogenesis.Nucleic acids research · 2018Article
- A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications.PloS one · 2016Article
- The Xenopus ORFeome: A resource that enables functional genomics.Developmental biology · 2015Article
- Modular assembly of transposon integratable multigene vectors using RecWay assembly.Nucleic acids research · 2013Article
- Construction of a highly flexible and comprehensive gene collection representing the ORFeome of the human pathogen Chlamydia pneumoniae.BMC genomics · 2012Article
- Efficient multicistronic co-expression of hNIS and hTPO in prostate cancer cells for nonthyroidal tumor radioiodine therapy.American journal of nuclear medicine and molecular imaging · 2012Article
- Article
- Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli.BMC microbiology · 2011Article
- Production of Myxoma virus gateway entry and expression libraries and validation of viral protein expression.Current protocols in microbiology · 2011Article
- ViralORFeome: an integrated database to generate a versatile collection of viral ORFs.Nucleic acids research · 2010Article
- Analysis of vaccinia virus-host protein-protein interactions: validations of yeast two-hybrid screenings.Journal of proteome research · 2009Article
- Modeling of the bacterial mechanism of methicillin-resistance by a systems biology approach.PloS one · 2009Article
- Global identification of protein kinase substrates by protein microarray analysis.Nature protocols · 2009Article
- Combination chemical genetics.Nature chemical biology · 2008Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe bacterium Staphylococcus aureus causes significant morbidity and mortality in humans, primarily due to the emergence of strains that are resistant to antibiotics - notably methicillin-resistant S. aureus (MRSA) isolates. Development of effective strategies for the control and treatment of MRSA infections may best be achieved through 'omics' approaches, which first requires cloning the entire set of S. aureus' protein-encoding open reading frames (ORFs), or ORFeome.
resultsThe complete genome sequence of S. aureus strain Mu50 has 2697 predicted protein-coding ORFs. Based on the sequence of this strain we designed PCR primers to construct from an S. aureus (non-MRSA) clinical isolate an ORFeome library that contains 2562 unique Gateway entry clones (95% coverage), each corresponding to a defined ORF. The high quality of the ORFeome library was verified by DNA sequencing and PCR amplification, and its functionality was demonstrated by expressing recombinant proteins and observing protein interactions in a yeast 2-hybrid homodimerization screen.
conclusionThis first ORFeome library for S. aureus provides an essential new tool for investigating the systems biology of this important pathogen.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.