ArticleJournal of lipid research2008
Fluorescence-topographic NSOM directly visualizes peak-valley polarities of GM1/GM3 rafts in cell membrane fluctuations.
Article in Journal of lipid research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Cell density-dependent membrane distribution of ganglioside GM3 in melanoma cells.Cellular and molecular life sciences : CMLS · 2023Article
- Sphingolipid metabolism and its relationship with cardiovascular, renal and metabolic diseases.Archivos de cardiologia de Mexico · 2023Review
- Differential lipidomics of HK-2 cells and exosomes under high glucose stimulation.International journal of medical sciences · 2022Article
- Glycocalyx Curving the Membrane: Forces Emerging from the Cell Exterior.Annual review of cell and developmental biology · 2021Review
- Comprehensive Lipidome Profiling of the Kidney in Early-Stage Diabetic Nephropathy.Frontiers in endocrinology · 2020Article
- Recent progress on lipid lateral heterogeneity in plasma membranes: From rafts to submicrometric domains.Progress in lipid research · 2016Review
- Changes in membrane sphingolipid composition modulate dynamics and adhesion of integrin nanoclusters.Scientific reports · 2016Article
- Sphingolipid Organization in the Plasma Membrane and the Mechanisms That Influence It.Frontiers in cell and developmental biology · 2016Review
- Ultrastructure and lipid composition of detergent-resistant membranes derived from mammalian sperm and two types of epithelial cells.Cell and tissue research · 2016Article
- There Is No Simple Model of the Plasma Membrane Organization.Frontiers in cell and developmental biology · 2016Review
- Nanoscale Relationship Between CD4 and CD25 of T Cells Visualized with NSOM/QD-Based Dual-Color Imaging System.Nanoscale research letters · 2015Article
- Differential uPAR recruitment in caveolar-lipid rafts by GM1 and GM3 gangliosides regulates endothelial progenitor cells angiogenesis.Journal of cellular and molecular medicine · 2015Article
- Current approaches to studying membrane organization.F1000Research · 2015Review
- Plasma membrane organization and function: moving past lipid rafts.Molecular biology of the cell · 2013Article
- Prominin-2 expression increases protrusions, decreases caveolae and inhibits Cdc42 dependent fluid phase endocytosis.Biochemical and biophysical research communications · 2013Article
- Direct chemical evidence for sphingolipid domains in the plasma membranes of fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Role of glycosphingolipids in dendritic cell-mediated HIV-1 trans-infection.Advances in experimental medicine and biology · 2013Review
- Nanoscale membrane organization: where biochemistry meets advanced microscopy.ACS chemical biology · 2012Review
- Membrane-bound IL-22 after de novo production in tuberculosis and anti-Mycobacterium tuberculosis effector function of IL-22+ CD4+ T cells.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Molecular insights into amyloid regulation by membrane cholesterol and sphingolipids: common mechanisms in neurodegenerative diseases.Expert reviews in molecular medicine · 2010Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Simultaneous fluorescence-topographic nanoscale imaging of cell-surface molecules in the context of membrane ultra-structures has not been reported. Here, near-field scanning optical microscopy (NSOM)-based direct fluorescence-topographic imaging indicated that GM3 rafts/nanodomains (190.0 +/- 49.8 nm ranging 84.5-365.0 nm) were localized predominantly on the peaks of microvillus-like protrusions in the apical membrane of GM3 + Madin-Darby canine kidney cells, whereas GM1 rafts/nanodomains (159.5 +/- 63.8 nm ranging 42-360 nm) were distributed mainly on the slops of protrusions or the valleys between protrusions in the plasma membranes of GM1 + MDCK cells. The data demonstrated that gangliosides polarized not only in a well-known apical-basolateral manner but also in the more microscopic peak-valley manner, implicating unique distribution of GM1 or GM3 in cell-surface fluctuations on the apical membrane of polarized cells. The peak-valley polarities of gangliosides also implicated their different functions relevant to lipid rafts, microvilli, or cellular processes. Importantly, our study demonstrated for the first time that the NSOM-based direct fluorescence-topographic imaging is unique and powerful for elucidating nanoscale distribution of specific cell-surface molecules in membrane fluctuations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.