ArticleJournal of controlled release : official journal of the Controlled Release Society2008
Triggered release of siRNA from poly(ethylene glycol)-protected, pH-dependent liposomes.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 104 citations in OpenAlex.
- Review
- Elucidation of lipid nanoparticle surface structure in mRNA vaccines.Scientific reports · 2023Article
- Isolation of Primary Mouse Lung Endothelial Cells.Journal of visualized experiments : JoVE · 2021Article
- Article
- Overcoming Barriers for siRNA Therapeutics: From Bench to Bedside.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Amphiphilic Polypeptides for VEGF siRNA Delivery into Retinal Epithelial Cells.Pharmaceutics · 2020Article
- Chemotherapeutic Drug Delivery and Quantitative Analysis of Proliferation, Apoptosis, and Migration in a Tissue-Engineered Three-Dimensional Microvessel Model of the Tumor Microenvironment.ACS biomaterials science & engineering · 2019Article
- Optimization of Magneto-thermally Controlled Release Kinetics by Tuning of Magnetoliposome Composition and Structure.Scientific reports · 2017Article
- Immune modulatory nanoparticle therapeutics for intracerebral glioma.Neuro-oncology · 2017Article
- Emerging applications of porphyrins in photomedicine.Frontiers in physics · 2015Article
- Aptamer-functionalized lipid nanoparticles targeting osteoblasts as a novel RNA interference-based bone anabolic strategy.Nature medicine · 2015Article
- siRNA Delivery by Stimuli-Sensitive Nanocarriers.Current pharmaceutical design · 2015Review
- Cationic lipid nanodisks as an siRNA delivery vehicle.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2014Article
- Catch and Release: Photocleavable Cationic Diblock Copolymers as a Potential Platform for Nucleic Acid Delivery.Polymer chemistry · 2014Article
- Recent In Vivo Evidences of Particle-Based Delivery of Small-Interfering RNA (siRNA) into Solid Tumors.Journal of pharmaceutical innovation · 2014Article
- Inhibiting metastatic breast cancer cell migration via the synergy of targeted, pH-triggered siRNA delivery and chemokine axis blockade.Molecular pharmaceutics · 2014Article
- Strategies on the nuclear-targeted delivery of genes.Journal of drug targeting · 2013Review
- Acid-degradable polymer-caged lipoplex (PCL) platform for siRNA delivery: facile cellular triggered release of siRNA.Journal of the American Chemical Society · 2013Article
- Stimuli-responsive nanocarriers for drug delivery.Nature materials · 2013Review
- Scalable method to produce biodegradable nanoparticles that rapidly penetrate human mucus.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
The ability of small interfering RNA (siRNA) to regulate gene expression has potential therapeutic applications, but its use is limited by inefficient delivery. Triggered release of adsorbed poly(ethylene glycol) (PEG)-b-polycation polymers from pH-dependent (PD) liposomes enables protection from immune recognition during circulation (pH 7.4) and subsequent intracellular delivery of siRNA within the endosome (pH ~5.5). Polycationic blocks, based on either poly[2-(dimethylamino)ethyl methacrylate] (31 or 62 DMA repeat units) or polylysine (21 K repeat units), act as anchors for a PEG (113 ethylene glycol repeat units) protective block. Incorporation of 1,2-dioleoyl-3-dimethylammonium-propane (DAP), a titratable lipid, increases the liposome's net cationic character within acidic environments, resulting in polymer desorption and membrane fusion. Liposomes encapsulating siRNA demonstrate green fluorescent protein (GFP) silencing in genetically-modified, GFP-expressing HeLa cells and glyceraldehyde-3-phosphate dehydrogenase (GAPD) knockdown in human umbilical vein endothelial cells (HUVEC). Bare and PD liposomes coated with PEG113-DMA31 exhibit a 0.16+/-0.2 and 0.32+/-0.3 fraction of GFP knockdown, respectively. In contrast, direct siRNA administration and Oligofectamine complexed siRNA reduce GFP expression by 0.06+/-0.02 and 0.14+/-0.02 fractions, respectively. Our in vitro data indicates that polymer desorption from PD liposomes enhances siRNA-mediated gene knockdown.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.