ArticleThe Journal of parasitology2009
Multiple displacement amplification for malaria parasite DNA.
Article in The Journal of parasitology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Extrachromosomal DNA amplicons in antimalarial-resistant Plasmodium falciparum.Molecular microbiology · 2021Article
- Optimization of whole-genome sequencing of Plasmodium falciparum from low-density dried blood spot samples.Malaria journal · 2021Article
- Utility of Plasmodium falciparum DNA from rapid diagnostic test kits for molecular analysis and whole genome amplification.Malaria journal · 2020Article
- Portable sequencer in the fight against infectious disease.Journal of human genetics · 2020Review
- Long term persistence of clonal malaria parasite Plasmodium falciparum lineages in the Colombian Pacific region.BMC genetics · 2013Article
- Using CF11 cellulose columns to inexpensively and effectively remove human DNA from Plasmodium falciparum-infected whole blood samples.Malaria journal · 2012Article
- Population genetic analysis of Plasmodium falciparum parasites using a customized Illumina GoldenGate genotyping assay.PloS one · 2011Article
- Evolutionary dynamics of the immunodominant repeats of the Plasmodium vivax malaria-vaccine candidate circumsporozoite protein (CSP).Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2010Article
- Drug resistance and genetic mapping in Plasmodium falciparum.Current genetics · 2008Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Multiple displacement amplification (MDA) using Phi29 has proved to be an efficient, high-fidelity method for whole genome amplification in many organisms. This project was designed to evaluate this approach for use with the malaria parasite Plasmodium falciparum. In particular, we were concerned that the AT richness and presence of contaminating human DNA could limit efficiency of MDA in this system. We amplified 60 DNA samples using phi29 and scored 14 microsatellites, 9 single-nucleotide polymorphisms (SNPs), and gene copy number at GTP-cyclohydrolase I both before and after MDA. We observed 100% concordance in 829 microsatellite genotypes and in 499 SNP genotypes. Furthermore, copy number estimates for the GTP-cyclohydrolase I gene were correlated (r(2) = 0.67) in pre- and postamplification samples. These data confirm that MDA permits scoring of a range of different types of polymorphisms in P. falciparum malaria and can be used to extend the life of valuable DNA stocks.
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Registered trials
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