ArticleJournal of biomedical optics
Energy migration alters the fluorescence lifetime of Cerulean: implications for fluorescence lifetime imaging Forster resonance energy transfer measurements.
Article in Journal of biomedical optics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Linking molecular tension and cellular tractions: a multiscale approach to focal adhesion mechanics.Communications biology · 2026Article
- Anomalous photophysical behaviors attributed to excitonic coupling in fluorescent proteins.Biophysical journal · 2025Article
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- Towards the Use of Individual Fluorescent Nanoparticles as Ratiometric Sensors: Spectral Robustness of Ultrabright Nanoporous Silica Nanoparticles.Sensors (Basel, Switzerland) · 2023Article
- KIR signaling is regulated by electrostatic interaction of its cytosolic tail with the plasma membrane despite being neutral polyampholyte.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Dynamics of HIV-1 Gag Processing as Revealed by Fluorescence Lifetime Imaging Microscopy and Single Virus Tracking.Viruses · 2022Article
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- A Guide to Fluorescence Lifetime Microscopy and Förster's Resonance Energy Transfer in Neuroscience.Current protocols in neuroscience · 2020Review
- QuanTI-FRET: a framework for quantitative FRET measurements in living cells.Scientific reports · 2020Article
- Development of heme protein based oxygen sensing indicators.Scientific reports · 2018Article
- Auto-FPFA: An Automated Microscope for Characterizing Genetically Encoded Biosensors.Scientific reports · 2018Article
- Measurement of intracellular concentration of fluorescently-labeled targets in living cells.PloS one · 2018Article
- Deciphering CaMKII Multimerization Using Fluorescence Correlation Spectroscopy and Homo-FRET Analysis.Biophysical journal · 2017Article
- Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Signal Improvement Strategies for Fluorescence Detection of Biomacromolecules.Journal of fluorescence · 2016Review
- Simultaneous FRAP, FLIM and FAIM for measurements of protein mobility and interaction in living cells.Biomedical optics express · 2015Article
- A single amino acid change in inhibitory killer cell Ig-like receptor results in constitutive receptor self-association and phosphorylation.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Functional fluorescent protein insertions in herpes simplex virus gB report on gB conformation before and after execution of membrane fusion.PLoS pathogens · 2014Article
- Application of phasor plot and autofluorescence correction for study of heterogeneous cell population.Journal of biomedical optics · 2014Article
- Measuring protein interactions using Förster resonance energy transfer and fluorescence lifetime imaging microscopy.Methods (San Diego, Calif.) · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
Abstract
Forster resonance energy transfer (FRET) is a physical phenomenon used to study molecular interactions in living cells. Changes in the fluorescence lifetime of proteins genetically tagged with a donor fluorophore, such as cyan fluorescent protein or Cerulean, are used to measure energy transfer to a protein tagged with an acceptor fluorophore (yellow fluorescent protein or Venus). Increased transfer efficiency is usually interpreted as closer proximity. Resonance energy transfer is also possible between identical fluorophores. This form of FRET is called energy migration resonance energy transfer (EM-RET). Theoretically, EM-RET should not alter the lifetime or emission spectrum measured from a population of fluorophores. We find a change in the fluorescent lifetime of Cerulean that correlates with energy migration and can result in significant errors when using Cerulean as a donor to measure FRET efficiencies based on fluorescence lifetimes [corrected]
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