ArticleJournal of biomedical optics
Characterization of an improved donor fluorescent protein for Forster resonance energy transfer microscopy.
Article in Journal of biomedical optics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 84 citations in OpenAlex.
- Article
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- Theory for High-Throughput Genetic Interaction Screening.ACS synthetic biology · 2023Article
- Visualizing Neurons Under Tension In Vivo with Optogenetic Molecular Force Sensors.Methods in molecular biology (Clifton, N.J.) · 2023Article
- An L-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR.Nature communications · 2021Article
- Nesprins are mechanotransducers that discriminate epithelial-mesenchymal transition programs.The Journal of cell biology · 2020Article
- Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse.eLife · 2020Article
- A STING-based biosensor affords broad cyclic dinucleotide detection within single living eukaryotic cells.Nature communications · 2020Article
- Enhanced Molecular Tension Sensor Based on Bioluminescence Resonance Energy Transfer (BRET).ACS sensors · 2020Article
- Improving Quality, Reproducibility, and Usability of FRET-Based Tension Sensors.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Intraneuronal Tau Misfolding Induced by Extracellular Amyloid-β Oligomers.Journal of Alzheimer's disease : JAD · 2019Article
- Synergistic Ensemble of Optogenetic Actuators and Dynamic Indicators in Cell Biology.Molecules and cells · 2018Review
- Review
- SensorFRET: A Standardless Approach to Measuring Pixel-based Spectral Bleed-through and FRET Efficiency using Spectral Imaging.Scientific reports · 2017Article
- Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish.Nature communications · 2017Article
- Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation.The journal of physical chemistry letters · 2017Article
- Coordination between Intra- and Extracellular Forces Regulates Focal Adhesion Dynamics.Nano letters · 2017Article
- Coordinated integrin activation by actin-dependent force during T-cell migration.Nature communications · 2016Article
- Article
- Sumoylation of p35 modulates p35/cyclin-dependent kinase (Cdk) 5 complex activity.Neuromolecular medicine · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The genetically encoded fluorescent proteins (FP), used in combination with Forster resonance energy transfer (FRET) microscopy, provide the tools necessary for the direct visualization of protein interactions inside living cells. Typically, the Cerulean and Venus variants of the cyan and yellow FPs are used for FRET studies, but there are limitations to their use. Here, Cerulean and the newly developed monomeric Teal FP (mTFP) are compared as FRET donors for Venus using spectral and fluorescence lifetime measurements from living cells. The results demonstrate that when compared to Cerulean, mTFP has increased brightness, optimal excitation using the standard 458-nm laser line, increased photostability, and improved spectral overlap with Venus. In addition, the two-photon excitation and fluorescence lifetime characteristics are determined for mTFP. Together, these measurements indicate that mTFP is an excellent donor fluorophore for FRET studies, and that its use may improve the detection of interactions involving proteins that are difficult to express, or that need to be produced at low levels in cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.