ArticleJournal of bone and mineral metabolism2008
Changes in the expression of CD106, osteogenic genes, and transcription factors involved in the osteogenic differentiation of human bone marrow mesenchymal stem cells.
Article in Journal of bone and mineral metabolism, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 82 citations in OpenAlex.
- Biological properties of valve materials using RGD and EC.Open medicine (Warsaw, Poland) · 2025Article
- In Vitro Cell Surface Marker Expression on Mesenchymal Stem Cell Cultures does not Reflect Their Ex Vivo Phenotype.Stem cell reviews and reports · 2024Article
- Thy-1 knockdown promotes the osteogenic differentiation of GMSCs via the Wnt/β-catenin pathway.Journal of cellular and molecular medicine · 2023Article
- Osteoinductive and Osteogenic Capacity of Freeze-Dried Bovine Bone Compared to Deproteinized Bovine Bone Mineral Scaffold in Human Umbilical Cord Mesenchymal Stem Cell Culture: An In Vitro Study.European journal of dentistry · 2023Article
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- Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources.BMC veterinary research · 2021Article
- The Role of Melanotransferrin (CD228) in the regulation of the differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells (hBM-MSC).International journal of medical sciences · 2021Article
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- Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties.Scientific reports · 2019Article
- Review
- Different Methylation Patterns of RUNX2, OSX, DLX5 and BSP in Osteoblastic Differentiation of Mesenchymal Stem Cells.Cell journal · 2015Article
- Characterization and evaluation of mesenchymal stem cells derived from human embryonic stem cells and bone marrow.Cell and tissue research · 2014Article
- Depolarization alters phenotype, maintains plasticity of predifferentiated mesenchymal stem cells.Tissue engineering. Part A · 2013Article
- Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro.Stem cell research & therapy · 2013Article
- Isolation and characterization of ovine mesenchymal stem cells derived from peripheral blood.BMC veterinary research · 2012Article
- Markers for characterization of bone marrow multipotential stromal cells.Stem cells international · 2012Article
- Zoledrinic Acid Induces Steoblastic Differentiation of Mesenchymal Stem Cells without Change in Hypomethylation Status of OSTERIX Promoter.Cell journal · 2012Article
- Development of novel monoclonal antibodies that define differentiation stages of human stromal (mesenchymal) stem cells.Molecules and cells · 2011Article
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stem cells (MSCs) are well known to possess multipotential differentiation and are becoming a good tool for clinical research. However, specific markers for their purification and the mechanism of their osteogenic differentiation remain to be elucidated. In the present study, we compared the expression of CD106, and osteogenic differentiation-related proteins and genes in human bone marrow (BM)-derived MSCs, before and after differentiation by FACS, histochemical staining, immunohistochemical staining, RT-PCR, and real-time PCR. It was found that MSCs were positive for CD13, CD29, CD44, CD73, CD90, CD105, and CD166, but negative for CD14, CD31, CD34, CD62E, CD45, and GlyA. Notably, CD106 was detected before osteogenic induction, but its expression was downregulated 10 fold after 2 weeks of osteogenic differentiation as determined by flow cytometry. The results of RT-PCR and real-time PCR revealed that the expression of CD106 mRNA in MSCs significantly decreased by 7.1-, 4.2-, and 5.1-fold, respectively after osteogenic, chondrogenic, and adipogenic differentiation. In contrast, other MSC-positive markers described above did not change significantly even after differentiation. Compared to levels in control cells, after 2 weeks of osteogenic differentiation, mRNA levels of alkaline phosphatase, bone sialoprotein, osteocalcin, and transcript factors RUNX2 and Osterix showed more than 2-fold, 5-fold, 1.5-fold, 2-fold, and 5-fold increase, respectively. Thus, we speculate that CD106 might be a useful surface marker for BMMSCs. Moreover, alkaline phosphatase, type I collagen, osteonectin, osteopontin, and biglycin were expressed in the early stages of osteogenic differentiation before bone sialoprotein and osteocalcin. The present study should help to provide a novel marker for isolating purified MSCs and characterizing osteogenic differentiation.
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