ArticleNature2008
Minimally invasive high-speed imaging of sarcomere contractile dynamics in mice and humans.
Article in Nature, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 161 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
161 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Systematic review of skeletal muscle passive mechanics experimental methodology.Journal of biomechanics · 2021Pooled it
- Is my model good enough? Best practices for verification and validation of musculoskeletal models and simulations of movement.Journal of biomechanical engineering · 2015Guideline
- Integrated Opto-Biomechatronics For Single Muscle Fibre Structure-Function Assessment: The MyoRobot 3.0.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Spiral scan and cylindrical deconvolution to maximize image volume and contrast of multiphoton GRIN microendoscopy.Biomedical optics express · 2026Article
- Isometric slow- and fast-contracting skeletal muscle fibres have more similar cellular and molecular properties at 37°C than at 25°C in older adults.The Journal of physiology · 2026Article
- Cell-MICS: Detecting Immune Cells With Label-Free Two-Photon Autofluorescence and Deep Learning.Journal of biophotonics · 2026Article
- Adaptations in biceps femoris long-head muscle-tendon mechanics during the Nordic hamstring exercise in response to 9 weeks of training.Journal of sport and health science · 2026Article
- Plectin affects cell viscoelasticity at small and large deformations.Biophysical journal · 2026Article
- Embedding muscle fibers in hydrogel improves viability and preserves contractile function during prolonged ex vivo culture.The Journal of general physiology · 2026Article
- Neuromechanical basis of region-specific differences and their implications for sport performance and injury prevention: a narrative review.European journal of applied physiology · 2025Review
- Hamstring Injury Mechanisms and Eccentric Training-Induced Muscle Adaptations: Current Insights and Future Directions.Sports medicine (Auckland, N.Z.) · 2025Review
- Embedding muscle fibers in hydrogel improves viability and preserves contractile function during prolongedbioRxiv : the preprint server for biology · 2025Article
- Muscle growth by sarcomere divisions.Science advances · 2025Article
- Pressure Between Residual Limb and Transtibial Prosthesis Socket Considering Muscle Contraction: A Finite Element Study.Annals of biomedical engineering · 2025Article
- Achieving optical transparency in live animals with absorbing molecules.Science (New York, N.Y.) · 2024Article
- Kinesin-14 HSET and KlpA are non-processive microtubule motors with load-dependent power strokes.Nature communications · 2024Article
- Fast myosin binding protein C knockout in skeletal muscle alters length-dependent activation and myofilament structure.Communications biology · 2024Article
- Optimal fibre length and maximum isometric force are the most influential parameters when modelling muscular adaptations to unloading using Hill-type muscle models.Frontiers in physiology · 2024Article
- An optimized approach to study nanoscale sarcomere structure utilizing super-resolution microscopy with nanobodies.PloS one · 2024Article
- Fast myosin binding protein C knockout in skeletal muscle alters length-dependent activation and myofilament structure.bioRxiv : the preprint server for biology · 2023Article
101 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Sarcomeres are the basic contractile units of striated muscle. Our knowledge about sarcomere dynamics has primarily come from in vitro studies of muscle fibres and analysis of optical diffraction patterns obtained from living muscles. Both approaches involve highly invasive procedures and neither allows examination of individual sarcomeres in live subjects. Here we report direct visualization of individual sarcomeres and their dynamical length variations using minimally invasive optical microendoscopy to observe second-harmonic frequencies of light generated in the muscle fibres of live mice and humans. Using microendoscopes as small as 350 microm in diameter, we imaged individual sarcomeres in both passive and activated muscle. Our measurements permit in vivo characterization of sarcomere length changes that occur with alterations in body posture and visualization of local variations in sarcomere length not apparent in aggregate length determinations. High-speed data acquisition enabled observation of sarcomere contractile dynamics with millisecond-scale resolution. These experiments point the way to in vivo imaging studies demonstrating how sarcomere performance varies with physical conditioning and physiological state, as well as imaging diagnostics revealing how neuromuscular diseases affect contractile dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.