ArticleBiophysical journal2008
Tuning the formation and rupture of single ligand-receptor bonds by hyaluronan-induced repulsion.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 30 citations in OpenAlex.
- Combining DNA scaffolds and acoustic force spectroscopy to characterize individual protein bonds.Biophysical journal · 2023Article
- Controlling T cells spreading, mechanics and activation by micropatterning.Scientific reports · 2021Article
- Nanobody-CD16 Catch Bond Reveals NK Cell Mechanosensitivity.Biophysical journal · 2019Article
- A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation.Scientific reports · 2016Article
- Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin.Scientific reports · 2015Article
- Kinetics and mechanics of two-dimensional interactions between T cell receptors and different activating ligands.Biophysical journal · 2012Article
- Article
- Minimal encounter time and separation determine ligand-receptor binding in cell adhesion.Biophysical journal · 2011Article
- Biomolecule association rates do not provide a complete description of bond formation.Biophysical journal · 2009Article
- Studying Molecular Interactions at the Single Bond Level with a Laminar Flow Chamber.Cellular and molecular bioengineering · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used a combination of laminar flow chamber and reflection interference microscopy to study the formation and rupture of single bonds formed between Fc-ICAM-1 attached to a substrate and anti-ICAM-1 carried by micrometric beads in the presence of a repulsive hyaluronan (HA) layer adsorbed onto the substrate. The absolute distance between the colloids and the surface was measured under flow with an accuracy of a few nanometers. We could verify the long-term prediction of classical lubrication theory for the movement of a sphere near a wall in a shear flow. The HA polymer layer exerted long-range repulsive steric force on the beads and the hydrodynamics at the boundary remained more or less unchanged. By incubating HA at various concentrations, the thickness of the layer, as estimated by beads most probable height, was tuned in the range 20-200 nm. Frequency of bond formation was decreased by more than one order of magnitude by increasing the thickness of the repulsive layer, while the lifetime of individual bonds was not affected. This study opens the way for further quantitative studies of the effect of molecular environment and separation distance on ligand-receptor association and dissociation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.