ArticleBiophysical journal2008
Stabilization provided by neighboring strands is critical for the mechanical stability of proteins.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Chemical Evolution of Early Macromolecules: From Prebiotic Oligopeptides to Self-Organizing Biosystems via Amyloid Formation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Prediction of the Stability of Protein Substructures Using AI/ML Techniques.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Effects of ligand binding on the mechanical stability of protein GB1 studied by steered molecular dynamics simulation.Journal of molecular modeling · 2016Article
- Nanomechanics of β-rich proteins related to neuronal disorders studied by AFM, all-atom and coarse-grained MD methods.Journal of molecular modeling · 2014Article
- Article
- Single-molecule studies on PolySUMO proteins reveal their mechanical flexibility.Biophysical journal · 2013Article
- The molecular mechanism underlying mechanical anisotropy of the protein GB1.Biophysical journal · 2012Article
- Mechanically untying a protein slipknot: multiple pathways revealed by force spectroscopy and steered molecular dynamics simulations.Journal of the American Chemical Society · 2012Article
- Low folding cooperativity of HP35 revealed by single-molecule force spectroscopy and molecular dynamics simulation.Biophysical journal · 2012Article
- GFP's mechanical intermediate states.PloS one · 2012Article
- Terminal amino acids disturb xylanase thermostability and activity.The Journal of biological chemistry · 2011Article
- Mechanical unfolding of acylphosphatase studied by single-molecule force spectroscopy and MD simulations.Biophysical journal · 2010Article
- Computational and single-molecule force studies of a macro domain protein reveal a key molecular determinant for mechanical stability.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Multiple Unfolding Intermediates Obtained by Molecular Dynamic Simulations under Stretching for Immunoglobulin-Binding Domain of Protein G.The open biochemistry journal · 2009Article
- The effect of different force applications on the protein-protein complex Barnase-Barstar.Biophysical journal · 2009Article
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Authors and funding
6 authors.
Funding
Abstract
Single-molecule force spectroscopy studies and steered molecular dynamics simulations have revealed that protein topology and pulling geometry play important roles in determining the mechanical stability of proteins. Most studies have focused on local interactions that are associated with the force-bearing beta-strands. Interactions mediated by neighboring strands are often overlooked. Here we use Top7 and barstar as model systems to illustrate the critical importance of the stabilization effect provided by neighboring beta-strands on the mechanical stability. Using single-molecule atomic force microscopy, we showed that Top7 and barstar, which have similar topology in their force-bearing region, exhibit vastly different mechanical-stability characteristics. Top7 is mechanically stable and unfolds at approximately 150 pN, whereas barstar is mechanically labile and unfolds largely below 50 pN. Steered molecular dynamics simulations revealed that stretching force peels one force-bearing strand away from barstar to trigger unfolding, whereas Top7 unfolds via a substructure-sliding mechanism. This previously overlooked stabilization effect from neighboring beta-strands is likely to be a general mechanism in protein mechanics and can serve as a guideline for the de novo design of proteins with significant mechanical stability and novel protein topology.
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