ArticleInvestigative ophthalmology & visual science2009
Characterization of a transient TCF/LEF-responsive progenitor population in the embryonic mouse retina.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Developmental genome-wide occupancy analysis of bZIP transcription factor NRL uncovers the role of c-Jun in early differentiation of rod photoreceptors in the mammalian retina.Human molecular genetics · 2022Article
- Cell fate decisions, transcription factors and signaling during early retinal development.Progress in retinal and eye research · 2022Review
- The peripheral eye: A neurogenic area with potential to treat retinal pathologies?Progress in retinal and eye research · 2019Review
- Spatiotemporal dynamics of canonical Wnt signaling during embryonic eye development and posterior capsular opacification (PCO).Experimental eye research · 2018Article
- Hippo Signaling Circuit and Divergent Tissue Growth in Mammalian Eye.Molecules and cells · 2018Review
- Differential Expression of NF2 in Neuroepithelial Compartments Is Necessary for Mammalian Eye Development.Developmental cell · 2018Article
- Loss of Axin2 Causes Ocular Defects During Mouse Eye Development.Investigative ophthalmology & visual science · 2016Article
- A trans-Regulatory Code for the Forebrain Expression of Six3.2 in the Medaka Fish.The Journal of biological chemistry · 2015Article
- Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/β-CATENIN SIGNALING PATHWAY.The Journal of biological chemistry · 2015Article
- Wnt ligands from the embryonic surface ectoderm regulate 'bimetallic strip' optic cup morphogenesis in mouse.Development (Cambridge, England) · 2015Article
- Multiple requirements of the focal dermal hypoplasia gene porcupine during ocular morphogenesis.The American journal of pathology · 2015Article
- Foxg1 is required to limit the formation of ciliary margin tissue and Wnt/β-catenin signalling in the developing nasal retina of the mouse.Developmental biology · 2013Article
- Article
- BCL2L1 (BCL-X) promotes survival of adult and developing retinal ganglion cells.Molecular and cellular neurosciences · 2012Article
- Expression of brain-derived neurotrophic factor is regulated by the Wnt signaling pathway.Neuroreport · 2012Article
- A re-evaluation of two key reagents for in vivo studies of Wnt signaling.Developmental dynamics : an official publication of the American Association of Anatomists · 2011Article
- Understanding the role of growth factors in embryonic development: insights from the lens.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Combinatorial regulation of optic cup progenitor cell fate by SOX2 and PAX6.Development (Cambridge, England) · 2011Article
- The expression of Wnt2b in the optic cup lip requires a border between the pigmented and nonpigmented epithelium.Molecular vision · 2010Article
- Ectopic Mitf in the embryonic chick retina by co-transfection of β-catenin and Otx2.Investigative ophthalmology & visual science · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeHigh mobility group (HMG) transcription factors of the T-cell-specific transcription factor/lymphoid enhancer binding factor (TCF/LEF) family are a class of intrinsic regulators that are dynamically expressed in the embryonic mouse retina. Activation of TCF/LEFs is a hallmark of the Wnt/beta-catenin pathway; however, the requirement for Wnt/beta-catenin and noncanonical Wnt signaling during mammalian retinal development remains unclear. The goal of the study was to characterize more fully a TCF/LEF-responsive retinal progenitor population in the mouse embryo and to correlate this with Wnt/beta-catenin signaling.
methodsTCF/LEF activation was analyzed in the TOPgal (TCF optimal promoter) reporter mouse at embryonic ages and compared to Axin2 mRNA expression, an endogenous readout of Wnt/beta-catenin signaling. Reporter expression was also examined in embryos with a retina-specific deletion of the beta-catenin gene (Ctnnb1), using Six3-Cre transgenic mice. Finally, the extent to which TOPgal cells coexpress cell cycle proteins, basic helix-loop-helix (bHLH) transcription factors, and other retinal cell markers was tested by double immunohistochemistry.
resultsTOPgal reporter activation occurred transiently in a subpopulation of embryonic retinal progenitor cells. Axin2 was not expressed in the central retina, and TOPgal reporter expression persisted in the absence of beta-catenin. Although a proportion of TOPgal-labeled cells were proliferative, most coexpressed the cyclin-dependent kinase inhibitor p27/Kip1.
conclusionsTOPgal cells give rise to the four earliest cell types: ganglion, amacrine, horizontal, and photoreceptor. TCF/LEF activation in the central retina does not correlate with Wnt/beta-catenin signaling, pointing to an alternate role for this transcription factor family during retinal development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.