ArticleProceedings of the National Academy of Sciences of the United States of America2008
An in vivo gene deletion system for determining temporal requirement of bacterial virulence factors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
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- New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila.Molecular & cellular proteomics : MCP · 2022Article
- Controlled Activity of themBio · 2017Article
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- csrR, a Paralog and Direct Target of CsrA, Promotes Legionella pneumophila Resilience in Water.mBio · 2015Article
- Inflammasome-mediated cell death in response to bacterial pathogens that access the host cell cytosol: lessons from legionella pneumophila.Frontiers in cellular and infection microbiology · 2013Review
- Control of gene expression in Helicobacter pylori using the Tet repressor.Journal of microbiological methods · 2013Article
- Induction of rapid cell death by an environmental isolate of Legionella pneumophila in mouse macrophages.Infection and immunity · 2013Article
- Cell biology of infection by Legionella pneumophila.Microbes and infection · 2013Review
- The protein SdhA maintains the integrity of the Legionella-containing vacuole.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Bacterial Type IV secretion systems: versatile virulence machines.Future microbiology · 2012Review
- Article
- Employing site-specific recombination for conditional genetic analysis in Sinorhizobium meliloti.Applied and environmental microbiology · 2011Article
- Legionella pneumophila LbtU acts as a novel, TonB-independent receptor for the legiobactin siderophore.Journal of bacteriology · 2011Article
- Comprehensive identification of protein substrates of the Dot/Icm type IV transporter of Legionella pneumophila.PloS one · 2011Article
- Targeting One of its Own: Expanding Roles of Substrates of the Legionella Pneumophila Dot/Icm Type IV Secretion System.Frontiers in microbiology · 2011Article
- Inhibition of host vacuolar H+-ATPase activity by a Legionella pneumophila effector.PLoS pathogens · 2010Article
- Targeting eEF1A by a Legionella pneumophila effector leads to inhibition of protein synthesis and induction of host stress response.Cellular microbiology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Analysis of phenotypes associated with specific mutants has been instrumental in determining the roles of a bacterial gene in a biological process. However, this technique does not allow one to address whether a specific gene or gene set is necessary to maintain such a process once it has been established. In the study of microbial pathogenesis, it is important but difficult to determine the temporal requirement of essential pathogenic determinants in the entire infection cycle. Here we report a Cre/loxP-based genetic system that allowed inducible deletion of specific bacterial genes after the pathogen had been phagocytosed by host cells. Using this system, we have examined the temporal requirement of the Dot/Icm type IV protein transporter of Legionella pneumophila during infection. We found that deletion of single essential dot/icm genes did not prevent the internalized bacteria from completing one cycle of intracellular replication. Further analyses indicate that the observed phenotypes were due to the high stability of the examined Dot/Icm protein. However, postinfection deletion within 8 h of the gene coding for the Dot/Icm substrate, SdhA, abolishes intracellular bacterial growth. This result indicates that the Dot/Icm transporter is important for intracellular bacterial growth after the initial biogenesis of the vacuole. Our study has provided a technical concept for analyzing the temporal requirement of specific bacterial proteins or protein complexes in infection or development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.