ArticleJournal of experimental & clinical cancer research : CR2008
CSE1L/CAS, the cellular apoptosis susceptibility protein, enhances invasion and metastasis but not proliferation of cancer cells.
Article in Journal of experimental & clinical cancer research : CR, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- CSE1L/CAS regulates cell proliferation through CDK signalling in mouse spermatogenesis.Cell proliferation · 2022Article
- Role of CSE1L expression in determining recurrence and survival of laryngeal tumors.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2022Article
- Interfering with CSE1L/CAS inhibits tumour growth via C3 in triple-negative breast cancer.Cell proliferation · 2022Article
- Cellular apoptosis susceptibility protein (CAS) suppresses the proliferation of breast cancer cells by upregulated cyp24a1.Medical oncology (Northwood, London, England) · 2020Article
- Expression of cellular apoptosis susceptibility (CAS) in the human testis and testicular germ cell tumors.Medical oncology (Northwood, London, England) · 2019Article
- Article
- Roles of the CSE1L-mediated nuclear import pathway in epigenetic silencing.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- CSE1L interaction with MSH6 promotes osteosarcoma progression and predicts poor patient survival.Scientific reports · 2017Article
- CAS (CSE1L) signaling pathway in tumor progression and its potential as a biomarker and target for targeted therapy.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Review
- Quantitative analysis of gene expression in fixed colorectal carcinoma samples as a method for biomarker validation.Molecular medicine reports · 2016Article
- Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage.Journal of translational medicine · 2013Article
- CSE1L, a novel microvesicle membrane protein, mediates Ras-triggered microvesicle generation and metastasis of tumor cells.Molecular medicine (Cambridge, Mass.) · 2012Article
- CSE1L, DIDO1 and RBM39 in colorectal adenoma to carcinoma progression.Cellular oncology (Dordrecht, Netherlands) · 2012Article
- Differential distributions of CSE1L/CAS and E-cadherin in the polarized and non-polarized epithelial glands of neoplastic colorectal epithelium.Journal of molecular histology · 2010Article
- Gene dysregulations driven by somatic copy number aberrations-biological and clinical implications in colon tumors: a paper from the 2009 William Beaumont Hospital Symposium on Molecular Pathology.The Journal of molecular diagnostics : JMD · 2010Review
- Cellular apoptosis susceptibility (CSE1L/CAS) protein in cancer metastasis and chemotherapeutic drug-induced apoptosis.Journal of experimental & clinical cancer research : CR · 2010Review
- Serum cellular apoptosis susceptibility protein is a potential prognostic marker for metastatic colorectal cancer.The American journal of pathology · 2010Article
- Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon.Molecular and cellular biochemistry · 2009Article
- The role of CSE1L expression in cervical lymph node metastasis of larynx tumors.Brazilian journal of otorhinolaryngologyArticle
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe cellular apoptosis susceptibility (CAS) protein is regarded as a proliferation-associated protein that associates with tumour proliferation as it associates with microtubule and functions in the mitotic spindle checkpoint. However, there is no any actual experimental study showing CAS (or CSE1 and CSE1L) can increase the proliferation of cancer cells. Previous pathological study has reported that CAS was strongly positive stained in all of the metastasis melanoma that be examined. Thus, CAS may regulate the invasion and metastasis of cancers. CAS is highly expressed in cancers; if CAS is associated with cancer proliferation, then increased CAS expression should be able to increase the proliferation of cancer cells. We studied whether increased CAS expression can increase cancer cell proliferation and whether CAS regulates the invasion of cancer cells.
methodsWe enhanced or reduced CAS expression by transfecting CAS or anti-CAS expression vectors into human MCF-7 breast cancer cells. The proliferations of cells were determined by trypan blue exclusion assay and flow cytometry analysis. Invasion of cancer cells were determined by matrigel-based invasion assay.
resultsOur studies showed that increased CAS expression was unable to enhance cancer cell proliferation. Immunofluorescence showed CAS was distributed in cytoplasm areas near cell membrane and cell protrusions. CAS was localized in cytoplasmic vesicle and immunogold electronmicroscopy showed CAS was located in vesicle membrane. CAS overexpression enhanced matrix metalloproteinase-2 (MMP-2) secretion and cancer cell invasion. Animal experiments showed CAS reduction inhibited the metastasis of B16-F10 melanoma cells by 56% in C57BL/6 mice.
conclusionOur results indicate that CAS increases the invasion but not the proliferation of cancer cells. Thus, CAS plus ECM-degradation proteinases may be used as the markers for predicting the advance of tumour metastasis.
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