ArticlePlant molecular biology2008
Introns control expression of sucrose transporter LeSUT1 in trichomes, companion cells and in guard cells.
Article in Plant molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 60 citations in OpenAlex.
- Immunohistochemical Localization of Proteins in the Phloem: Problems and Solutions.Methods in molecular biology (Clifton, N.J.) · 2026Article
- How pollen tubes fight for food: the impact of sucrose carriers and invertases ofFrontiers in plant science · 2023Article
- Genomic editing of intronic enhancers unveils their role in fine-tuning tissue-specific gene expression in Arabidopsis thaliana.The Plant cell · 2021Article
- Expression of Sucrose Transporters fromInternational journal of molecular sciences · 2020Article
- Role of blue and red light in stomatal dynamic behaviour.Journal of experimental botany · 2020Review
- Genome-wide association study revealed that the TaGW8 gene was associated with kernel size in Chinese bread wheat.Scientific reports · 2019Article
- Tissue-specific transcriptomic profiling of Plantago major provides insights for the involvement of vasculature in phosphate deficiency responses.Molecular genetics and genomics : MGG · 2019Article
- Molecular Evolution and Interaction of Membrane Transport and Photoreception in Plants.Frontiers in genetics · 2019Review
- Article
- Protoplast-Esculin Assay as a New Method to Assay Plant Sucrose Transporters: Characterization of AtSUC6 and AtSUC7 Sucrose Uptake Activity in Arabidopsis Col-0 Ecotype.Frontiers in plant science · 2018Article
- The RopGEF2-ROP7/ROP2 Pathway Activated by phyB Suppresses Red Light-Induced Stomatal Opening.Plant physiology · 2017Article
- Suppression of sucrose synthase affects auxin signaling and leaf morphology in tomato.PloS one · 2017Article
- Intronic Sequence Regulates Sugar-Dependent Expression of Arabidopsis thaliana Production of Anthocyanin Pigment-1/MYB75.PloS one · 2016Article
- Diverse expression of sucrose transporter gene family in Zea mays.Journal of genetics · 2015Article
- The guard cell metabolome: functions in stomatal movement and global food security.Frontiers in plant science · 2015Review
- Article
- Functional analysis of a rice late pollen-abundant UDP-glucose pyrophosphorylase (OsUgp2) promoter.Molecular biology reports · 2011Article
- Characterization of the phosphate starvation-induced glycerol-3-phosphate permease gene family in Arabidopsis.Plant physiology · 2011Article
- Evidence for a DNA-Based Mechanism of Intron-Mediated Enhancement.Frontiers in plant science · 2011Article
- Testing the IMEter on rice introns and other aspects of intron-mediated enhancement of gene expression.Journal of experimental botany · 2011Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In solanaceous plants such as tomato and tobacco, the sucrose transporter SUT1 is crucial for phloem loading. Using GUS as a reporter, the promoter and other regulatory cis elements required for the tomato LeSUT1 expression were analyzed by heterologous expression of translational chimeric constructs in tobacco. Although LeSUT1 is highly expressed at the RNA level, GUS expression under the control of a 1.8 kb LeSUT1 promoter resulted in few plants expressing GUS. In GUS-positive transformants, expression levels were low and limited to leaf phloem. Increasing or decreasing the length of LeSUT1 promoter did not lead to a significant increase in positive transformants or higher expression levels. Translational fusion of GUS to the LeSUT1 C-terminus in a construct containing all exons and introns and the 3'-UTR led to a higher number of positive transformants and many plants with high GUS activity. LeSUT1 expression was detected in ab- and adaxial phloem companion cells, trichomes and guard cells. The role of individual introns in LeSUT1 expression was further analyzed by placing each LeSUT1 intron into the 5'-UTR within the 2.3 kb LeSUT1 promoter construct. Results showed remarkable functions for the three introns for SUT1 expression in trichomes, guard cells and phloem cells. Intron 3 is responsible for expression in trichomes, whereas intron 2 is necessary for expression in companion cells and guard cells. The combination of all introns is required for the full expression pattern in phloem, guard cells and trichomes.
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