ArticleRNA (New York, N.Y.)2008
Spliceosomal snRNAs in the unicellular eukaryote Trichomonas vaginalis are structurally conserved but lack a 5'-cap structure.
Article in RNA (New York, N.Y.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Recent advances in the molecular biology of the protist parasiteFaculty reviews · 2021Review
- An in vitro characterisation of the Trichomonas vaginalis TATA box-binding proteins (TBPs).Parasitology research · 2019Article
- The Integrator complex regulates differential snRNA processing and fate of adult stem cells in the highly regenerative planarian Schmidtea mediterranea.PLoS genetics · 2018Article
- Spliceosomal introns in Trichomonas vaginalis revisited.Parasites & vectors · 2018Article
- CRISPR/Cas9-mediated gene modification and gene knock out in the human-infective parasite Trichomonas vaginalis.Scientific reports · 2018Article
- The parasite Trichomonas vaginalis expresses thousands of pseudogenes and long non-coding RNAs independently from functional neighbouring genes.BMC genomics · 2014Article
- A trans-spliced telomerase RNA dictates telomere synthesis in Trypanosoma brucei.Cell research · 2013Article
- Getting trichy: tools and approaches to interrogating Trichomonas vaginalis in a post-genome world.Trends in parasitology · 2013Review
- Evolutionarily divergent spliceosomal snRNAs and a conserved non-coding RNA processing motif in Giardia lamblia.Nucleic acids research · 2012Article
- Box H/ACA snoRNAs are preferred substrates for the trimethylguanosine synthase in the divergent unicellular eukaryote Trichomonas vaginalis.RNA (New York, N.Y.) · 2012Article
- Functional analysis of sequence motifs involved in the polyadenylation of Trichomonas vaginalis mRNAs.Eukaryotic cell · 2012Article
- Characterization of RNase MRP RNA and novel snoRNAs from Giardia intestinalis and Trichomonas vaginalis.BMC genomics · 2011Article
- Trichomoniasis: evaluation to execution.European journal of obstetrics, gynecology, and reproductive biology · 2011Review
- An essential role for trimethylguanosine RNA caps in Saccharomyces cerevisiae meiosis and their requirement for splicing of SAE3 and PCH2 meiotic pre-mRNAs.Nucleic acids research · 2011Article
- Characterizing ncRNAs in Human Pathogenic Protists Using High-Throughput Sequencing Technology.Frontiers in genetics · 2011Article
- A metazoan/plant-like capping enzyme and cap modified nucleotides in the unicellular eukaryote Trichomonas vaginalis.PLoS pathogens · 2010Article
- 5' and 3' end modifications of spliceosomal RNAs in Plasmodium falciparum.Molecular biology reports · 2010Article
- Regulation of gene expression in protozoa parasites.Journal of biomedicine & biotechnology · 2010Review
- High throughput genome-wide survey of small RNAs from the parasitic protists Giardia intestinalis and Trichomonas vaginalis.Genome biology and evolution · 2009Article
- The divergent eukaryote Trichomonas vaginalis has an m7G cap methyltransferase capable of a single N2 methylation.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Few genes in the divergent eukaryote Trichomonas vaginalis have introns, despite the unusually large gene repertoire of this human-infective parasite. These introns are characterized by extended conserved regulatory motifs at the 5' and 3' boundaries, a feature shared with another divergent eukaryote, Giardia lamblia, but not with metazoan introns. This unusual characteristic of T. vaginalis introns led us to examine spliceosomal small nuclear RNAs (snRNAs) predicted to mediate splicing reactions via interaction with intron motifs. Here we identify T. vaginalis U1, U2, U4, U5, and U6 snRNAs, present predictions of their secondary structures, and provide evidence for interaction between the U2/U6 snRNA complex and a T. vaginalis intron. Structural models predict that T. vaginalis snRNAs contain conserved sequences and motifs similar to those found in other examined eukaryotes. These data indicate that mechanisms of intron recognition as well as coordination of the two catalytic steps of splicing have been conserved throughout eukaryotic evolution. Unexpectedly, we found that T. vaginalis spliceosomal snRNAs lack the 5' trimethylguanosine cap typical of snRNAs and appear to possess unmodified 5' ends. Despite the lack of a cap structure, U1, U2, U4, and U5 genes are transcribed by RNA polymerase II, whereas the U6 gene is transcribed by RNA polymerase III.
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