ArticleMolecular biology of the cell2008
ZO-1- and ZO-2-dependent integration of myosin-2 to epithelial zonula adherens.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 80 citations in OpenAlex.
- PAK4 promotes vertex remodeling to maintain epithelial integrity and barrier function.bioRxiv : the preprint server for biology · 2025Article
- Cholesterol-rich domain formation mediated by ZO proteins is essential for tight junction formation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Claudins and JAM-A coordinately regulate tight junction formation and epithelial polarity.The Journal of cell biology · 2019Article
- The mechanobiology of tight junctions.Biophysical reviews · 2019Review
- ZO-2 Is a Master Regulator of Gene Expression, Cell Proliferation, Cytoarchitecture, and Cell Size.International journal of molecular sciences · 2019Review
- TheMolecular biology of the cell · 2019Article
- R40.76 binds to the α domain of ZO-1: role of ZO-1 (α+) in epithelial differentiation and mechano-sensing.Tissue barriers · 2019Article
- AMPK-dependent phosphorylation of cingulin reversibly regulates its binding to actin filaments and microtubules.Scientific reports · 2018Article
- Visualizing the dynamic coupling of claudin strands to the actin cytoskeleton through ZO-1.Molecular biology of the cell · 2017Article
- The mucosal barrier at a glance.Journal of cell science · 2017Review
- ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation.The Journal of cell biology · 2015Article
- Cell adhesion in epidermal development and barrier formation.Current topics in developmental biology · 2015Review
- Article
- JAM-A associates with ZO-2, afadin, and PDZ-GEF1 to activate Rap2c and regulate epithelial barrier function.Molecular biology of the cell · 2013Article
- ZO-1 recruitment to α-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions.Journal of cell science · 2013Article
- ARHGEF11, a regulator of junction-associated actomyosin in epithelial cells.Tissue barriers · 2013Article
- Protein kinase C-ζ mediates lung injury induced by diesel exhaust particles.American journal of respiratory cell and molecular biology · 2013Article
- A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin.PloS one · 2013Article
- Rho GTP exchange factor ARHGEF11 regulates the integrity of epithelial junctions by connecting ZO-1 and RhoA-myosin II signaling.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Intracellular mediators of JAM-A-dependent epithelial barrier function.Annals of the New York Academy of Sciences · 2012Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the zonula adherens (ZA) to be established by linear arrangement of adherens junctions (AJs) in epithelial sheet cells, critical for the epithelial cell sheet formation and intercellular barrier function, myosin-2 is supposedly integrated into the ZA with the result of overlapping localization of E-cadherin/actin/myosin-2. Here, we immunofluorescently showed that myosin-2 failed to be integrated into the ZA in cultured epithelial-type ZO1(ko)/2(kd) Eph4 cells lacking ZO-1 and -2 (zonula occludens-1 and -2) by knockout and knockdown, respectively. Instead, a linearized but fragmented arrangement of AJs was formed in the way that it was positive for E-cadherin/actin, but negative for myosin-2 (designated prezonula-AJ). Transfection of full-length ZO-1 or ZO-2, or ZO-1 lacking its PDZ (PSD-95/discs large/zonula occludens-1)-1/2 domains (but not one lacking PDZ-1/2/3) into ZO1(ko)/2(kd) Eph4 cells restored the junctional integration of myosin-2 with prezonula-AJ to establish the ZA. Transfection of dominant-active RhoA or Rho-kinase (ROCK), as well as administration of lysophosphatidic acid or Y27632, which activates RhoA or inhibits ROCK, respectively, suggested that RhoA regulated the junctional integration of myosin-2 into ZA in a manner such that ROCK played a necessary but not-sufficient role. Fluorescence resonance energy transfer analyses revealed that spatiotemporal Rho-activation occurred in a ZO-1/2-dependent way to establish ZA from primordial forms in epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.