ArticleAmerican journal of physiology. Cell physiology2008
Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct.
Article in American journal of physiology. Cell physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Tissue Distribution, Expression and Regulation of Urea Transporters.Sub-cellular biochemistry · 2025Review
- Membrane Dynamics Regulated by Cytoskeleton in Plant Immunity.International journal of molecular sciences · 2023Review
- α-Actinin 4 Links Vasopressin Short-Term and Long-Term Regulation of Aquaporin-2 in Kidney Collecting Duct Cells.Frontiers in physiology · 2021Article
- Lipidomic and proteomic analysis of exosomes from mouse cortical collecting duct cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Regulation of Transporters and Channels by Membrane-Trafficking Complexes in Epithelial Cells.Cold Spring Harbor perspectives in biology · 2017Review
- Impaired AQP2 trafficking in Fxyd1 knockout mice: A role for FXYD1 in regulated vesicular transport.PloS one · 2017Article
- Molecular mechanisms regulating aquaporin-2 in kidney collecting duct.American journal of physiology. Renal physiology · 2016Review
- The endoplasmic reticulum and casein-containing vesicles contribute to milk fat globule membrane.Molecular biology of the cell · 2016Article
- Cytoskeletal Components Define Protein Location to Membrane Microdomains.Molecular & cellular proteomics : MCP · 2015Article
- Vasopressin induces apical expression of caveolin in rat kidney collecting duct principal cells.American journal of physiology. Renal physiology · 2013Article
- Quantitative apical membrane proteomics reveals vasopressin-induced actin dynamics in collecting duct cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Dynamin2, clathrin, and lipid rafts mediate endocytosis of the apical Na/K/2Cl cotransporter NKCC2 in thick ascending limbs.The Journal of biological chemistry · 2012Article
- Molecular roles of Myo1c function in lipid raft exocytosis.Communicative & integrative biology · 2012Article
- Close association of aquaporin-2 internalization with caveolin-1.Acta histochemica et cytochemica · 2012Article
- Gene expression databases for kidney epithelial cells.American journal of physiology. Renal physiology · 2012Review
- Molecular regulation of NKCC2 in the thick ascending limb.American journal of physiology. Renal physiology · 2011Review
- Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.Molecular & cellular proteomics : MCP · 2011Article
- Constitutive endocytosis and recycling of NKCC2 in rat thick ascending limbs.American journal of physiology. Renal physiology · 2010Article
- Phosphoproteomic profiling reveals vasopressin-regulated phosphorylation sites in collecting duct.Journal of the American Society of Nephrology : JASN · 2010Article
- Galectin-3, a novel centrosome-associated protein, required for epithelial morphogenesis.Molecular biology of the cell · 2010Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
In the renal collecting duct, vasopressin controls transport of water and solutes via regulation of membrane transporters such as aquaporin-2 (AQP2) and the epithelial urea transporter UT-A. To discover proteins potentially involved in vasopressin action in rat kidney collecting ducts, we enriched membrane "raft" proteins by harvesting detergent-resistant membranes (DRMs) of the inner medullary collecting duct (IMCD) cells. Proteins were identified and quantified with LC-MS/MS. A total of 814 proteins were identified in the DRM fractions. Of these, 186, including several characteristic raft proteins, were enriched in the DRMs. Immunoblotting confirmed DRM enrichment of representative proteins. Immunofluorescence confocal microscopy of rat IMCDs with antibodies to DRM proteins demonstrated heterogeneity of raft subdomains: MAL2 (apical region), RalA (predominant basolateral labeling), caveolin-2 (punctate labeling distributed throughout the cells), and flotillin-1 (discrete labeling of large intracellular structures). The DRM proteome included GPI-anchored, doubly acylated, singly acylated, cholesterol-binding, and integral membrane proteins (IMPs). The IMPs were, on average, much smaller and more hydrophobic than IMPs identified in non-DRM-enriched IMCD. The content of serine 256-phosphorylated AQP2 was greater in DRM than in non-DRM fractions. Vasopressin did not change the DRM-to-non-DRM ratio of most proteins, whether quantified by tandem mass spectrometry (LC-MS/MS, n=22) or immunoblotting (n=6). However, Rab7 and annexin-2 showed small increases in the DRM fraction in response to vasopressin. In accord with the long-term goal of creating a systems-level analysis of transport regulation, this study has identified a large number of membrane-associated proteins expressed in the IMCD that have potential roles in vasopressin action.
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Registered trials
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