ArticleGenes & development2008
SpoIIIE strips proteins off the DNA during chromosome translocation.
Article in Genes & development, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 70 citations in OpenAlex.
- A safeguarding factor monitors septal pore stability to secure compartmentalization during sporulation in Bacillus subtilis.Communications biology · 2026Article
- Developmentally regulated proteolysis by MdfA and ClpCP mediates metabolic differentiation duringGenes & development · 2025Article
- Ribosomes translocation into the spore of Bacillus subtilis is highly organised and requires peptidoglycan rearrangements.Nature communications · 2025Article
- Clostridioides difficile Sporulation.Advances in experimental medicine and biology · 2024Review
- Chromosome Segregation and Peptidoglycan Remodeling Are Coordinated at a Highly Stabilized Septal Pore to Maintain Bacterial Spore Development.Developmental cell · 2021Article
- NFrontiers in microbiology · 2021Article
- Metabolic differentiation and intercellular nurturing underpin bacterial endospore formation.Science advances · 2021Article
- Milestones inMicrobial cell (Graz, Austria) · 2020Review
- Cohesion of Sister Chromosome Termini during the Early Stages of Sporulation in Bacillus subtilis.Journal of bacteriology · 2020Article
- Two-step chromosome segregation in the stalked budding bacterium Hyphomonas neptunium.Nature communications · 2019Article
- Article
- Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation.Scientific reports · 2018Article
- Mechanochemical coupling and bi-phasic force-velocity dependence in the ultra-fast ring ATPase SpoIIIE.eLife · 2018Article
- Sequential eviction of crowded nucleoprotein complexes by the exonuclease RecBCD molecular motor.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Specialized activities and expression differences for Clostridium thermocellum biofilm and planktonic cells.Scientific reports · 2017Article
- Kinetics of large-scale chromosomal movement during asymmetric cell division in Escherichia coli.PLoS genetics · 2017Article
- Single-Molecule Imaging Reveals a Collapsed Conformational State for DNA-Bound Cohesin.Cell reports · 2016Article
- Missense Mutations Allow a Sequence-Blind Mutant of SpoIIIE to Successfully Translocate Chromosomes during Sporulation.PloS one · 2016Article
- Two-subunit DNA escort mechanism and inactive subunit bypass in an ultra-fast ring ATPase.eLife · 2015Article
- Visualizing protein movement on DNA at the single-molecule level using DNA curtains.DNA repair · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The FtsK/SpoIIIE family of DNA transporters are responsible for translocating missegregated chromosomes after the completion of cell division. An extreme example of this post-cytokinetic DNA segregation occurs during spore formation in the bacterium Bacillus subtilis, where SpoIIIE pumps three-quarters of the chromosome (>3 megabases) into one of the two daughter cells. Here, we investigate the fate of the proteins associated with the translocated DNA. Taking advantage of several unique features of Bacillus sporulation, we demonstrate that RNA polymerase, transcription factors, and chromosome remodeling proteins are stripped off the DNA during translocation of the chromosome into the forespore compartment. Furthermore, we show that in vitro the soluble ATPase domain of SpoIIIE can displace RNA polymerase bound to DNA, suggesting that SpoIIIE alone is capable of this wire-stripping activity. Our data suggest that the bulk of the forespore chromosome is translocated naked into the forespore compartment. We propose that the translocation-stripping activity of SpoIIIE plays a key role in reprogramming developmental gene expression in the forespore.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.