ArticlePharmaceutical research2008
Microfabricated particles for engineered drug therapies: elucidation into the mechanisms of cellular internalization of PRINT particles.
Article in Pharmaceutical research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 95 citations in OpenAlex.
- Pharmacokinetics and tumor delivery of nanoparticles.Journal of drug delivery science and technology · 2023Article
- Cellulose Mediated Transferrin Nanocages for Enumeration of Circulating Tumor Cells for Head and Neck Cancer.Scientific reports · 2020Article
- Dual functional matrix metalloproteinase-responsive curcumin-loaded nanoparticles for tumor-targeted treatment.Drug delivery · 2019Article
- Co-exposure to polystyrene plastic beads and polycyclic aromatic hydrocarbon contaminants in fish gill (RTgill-W1) and intestinal (RTgutGC) epithelial cells derived from rainbow trout (Oncorhynchus mykiss).Environmental pollution (Barking, Essex : 1987) · 2019Article
- Peptide-Functionalized Hydrogel Cubes for Active Tumor Cell Targeting.Biomacromolecules · 2018Article
- Targeted PRINT Hydrogels: The Role of Nanoparticle Size and Ligand Density on Cell Association, Biodistribution, and Tumor Accumulation.Nano letters · 2015Article
- Novel targeted siRNA-loaded hybrid nanoparticles: preparation, characterization and in vitro evaluation.Journal of nanobiotechnology · 2015Article
- Rapid and Persistent Delivery of Antigen by Lymph Node Targeting PRINT Nanoparticle Vaccine Carrier To Promote Humoral Immunity.Molecular pharmaceutics · 2015Article
- Parameters and characteristics governing cellular internalization and trans-barrier trafficking of nanostructures.International journal of nanomedicine · 2015Review
- Development of functional biomaterials with micro- and nanoscale technologies for tissue engineering and drug delivery applications.Journal of tissue engineering and regenerative medicine · 2014Review
- Novel flower-shaped albumin particles as controlled-release carriers for drugs to penetrate the round-window membrane.International journal of nanomedicine · 2014Article
- Article
- Physical and chemical strategies for therapeutic delivery by using polymeric nanoparticles.Pharmacological reviews · 2012Review
- Enhancement of airway gene transfer by DNA nanoparticles using a pH-responsive block copolymer of polyethylene glycol and poly-L-lysine.Biomaterials · 2012Article
- Pulmonary drug delivery strategies: A concise, systematic review.Lung India : official organ of Indian Chest Society · 2012Article
- Article
- Nanocarriers for vascular delivery of antioxidants.Nanomedicine (London, England) · 2011Review
- More effective nanomedicines through particle design.Small (Weinheim an der Bergstrasse, Germany) · 2011Review
- Structure-property relationship in cytotoxicity and cell uptake of poly(2-oxazoline) amphiphiles.Journal of controlled release : official journal of the Controlled Release Society · 2011Article
- Hydrogel microparticles from lithographic processes: novel materials for fundamental and applied colloid science.Current opinion in colloid & interface science · 2011Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo investigate the cellular internalization pathways of shape- and size-specific particles as a function of zeta potential in different cell types.
methodsA top-down particle fabrication technique called PRINT was utilized to fabricate monodisperse 1 microm cylindrical particles. Cellular internalization of these PRINT particles was monitored using confocal microscopy, flow cytometry, and transmission electron microscopy. The endocytic pathway used by 1 microm cationic PRINT particles was evaluated using different inhibitory strategies. Cytotoxicity assays were used to determine the toxicity of both cationic and anionic PRINT particles in multiple cell types.
resultsParticle internalization was confirmed using confocal microscopy, flow cytometry and transmission electron microscopy. The mechanism of internalization of positively charged PRINT particles was found to be predominantly clathrin-mediated endocytosis and macropinocytosis with very few particles utilizing a caveolae-mediated endocytic pathway. The exposed charge on the surface of the particles had a significant effect on the rate of endocytosis in all cell types tested, except for the macrophage cells. No significant cytotoxicity was observed for all PRINT particles used in the present study.
conclusionsCylindrical 1 microm PRINT particles were readily internalized into HeLa, NIH 3T3, OVCAR-3, MCF-7, and RAW 264.7 cells. Particles with a positive zeta potential exhibited an enhanced rate of endocytosis compared to negatively charged particles with identical sizes and shapes. It was found that PRINT particles with a positive zeta potential were endocytosed into HeLa cells using predominantely clathrin-mediated and macropinocytotic pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.