ArticleGene expression2008
Mammalian Rrn3 is required for the formation of a transcription competent preinitiation complex containing RNA polymerase I.
Article in Gene expression, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- Rrn3 gene knockout affects ethanol-induced locomotion in adult heterozygous zebrafish.Psychopharmacology · 2022Article
- Review
- Conditional depletion of the RNA polymerase I subunit PAF53 reveals that it is essential for mitosis and enables identification of functional domains.The Journal of biological chemistry · 2019Article
- Molecular insight into RNA polymerase I promoter recognition and promoter melting.Nature communications · 2019Article
- Regulation of Ribosome Biogenesis in Skeletal Muscle Hypertrophy.Physiology (Bethesda, Md.) · 2019Review
- FGFR2 mutations in bent bone dysplasia syndrome activate nucleolar stress and perturb cell fate determination.Human molecular genetics · 2017Article
- Article
- TIF-IA: An oncogenic target of pre-ribosomal RNA synthesis.Biochimica et biophysica acta · 2016Review
- The Regulation of rRNA Gene Transcription during Directed Differentiation of Human Embryonic Stem Cells.PloS one · 2016Article
- Selective inhibition of rDNA transcription by a small-molecule peptide that targets the interface between RNA polymerase I and Rrn3.Molecular cancer research : MCR · 2014Article
- Architecture of the Saccharomyces cerevisiae RNA polymerase I Core Factor complex.Nature structural & molecular biology · 2014Article
- Characterization of the interactions of mammalian RNA polymerase I associated proteins PAF53 and PAF49.Biochemistry · 2012Article
- Regulation of ribosomal RNA production by RNA polymerase I: does elongation come first?Genetics research international · 2012Article
- Molecular basis of Rrn3-regulated RNA polymerase I initiation and cell growth.Genes & development · 2011Article
- TFIIB-related factors in RNA polymerase I transcription.Biochimica et biophysica actaReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mammalian Rrn3, an essential, polymerase-associated protein, is inactivated when cells are treated with cycloheximide, resulting in the inhibition of transcription by RNA polymerase I. Although Rrn3 is essential for transcription, its function in rDNA transcription has not been determined. For example, it is unclear whether Rrn3 is required for initiation or elongation by RNA polymerase I. Rrn3 has been shown to interact with the 43-kDa subunit of RNA polymerase I and with two of the subunits of SL1. In the current model for transcription, Rrn3 functions to recruit RNA polymerase I to the committed complex formed by SL1 and the rDNA promoter. To examine the question as to whether Rrn3 is required for the recruitment of RNA polymerase I to the template, we developed a novel assay similar to chromatin immunoprecipitation assays. We found that RNA polymerase I can be recruited to a template in the absence of active Rrn3. However, that complex will not initiate transcription, even after Rrn3 is added to the reaction. Interestingly, the complex that forms in the presence of active Rrn3 is biochemically distinguishable from that which forms in the absence of active Rrn3. For example, the functional complex is fivefold more resistant to heparin than that which forms in the absence of Rrn3. Our data demonstrate that Rrn3 must be present when the committed template complex is forming for transcription to occur.
Indexed as
Identifiers
18590050PMC2526047W2416845944What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.