ArticleBone2008
Heparanase expression and activity influences chondrogenic and osteogenic processes during endochondral bone formation.
Article in Bone, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Exploring Growth Pattern and Candidate Genes for Chicken Spur.Animals : an open access journal from MDPI · 2026Article
- Schwartz-Jampel Syndrome Type 1: Compound Heterozygosity of Two Novel VariantsJournal of clinical research in pediatric endocrinology · 2026Article
- The Role of Heparan Sulfate in Bone Repair and Regeneration.Calcified tissue international · 2025Review
- Modeling skeletal dysplasia in Hurler syndrome using patient-derived bone marrow osteoprogenitor cells.JCI insight · 2024Article
- Regulation of FGF-2, FGF-18 and Transcription Factor Activity by Perlecan in the Maturational Development of Transitional Rudiment and Growth Plate Cartilages and in the Maintenance of Permanent Cartilage Homeostasis.International journal of molecular sciences · 2022Review
- Perlecan in the Natural and Cell Therapy Repair of Human Adult Articular Cartilage: Can Modifications in This Proteoglycan Be a Novel Therapeutic Approach?Biomolecules · 2021Article
- Topical application of Heparanase-1 facilitates bone remodeling during the healing of bone defects in a mouse model.Journal of the Chinese Medical Association : JCMA · 2020Article
- Modular Proteoglycan Perlecan/Genes · 2018Review
- Identification of novel genetic variations affecting osteoarthritis patients.BMC medical genetics · 2016Article
- Single molecule force measurements of perlecan/HSPG2: A key component of the osteocyte pericellular matrix.Matrix biology : journal of the International Society for Matrix Biology · 2016Article
- Iroquois Proteins Promote Skeletal Joint Formation by Maintaining Chondrocytes in an Immature State.Developmental cell · 2015Article
- Heparanase stimulates chondrogenesis and is up-regulated in human ectopic cartilage: a mechanism possibly involved in hereditary multiple exostoses.The American journal of pathology · 2015Article
- The Schwartz-Jampel syndrome: Case report and review of literature.Advanced biomedical research · 2015Article
- Deficiency in perlecan/HSPG2 during bone development enhances osteogenesis and decreases quality of adult bone in mice.Calcified tissue international · 2014Article
- Transcriptional activation by NFκB increases perlecan/HSPG2 expression in the desmoplastic prostate tumor microenvironment.Journal of cellular biochemistry · 2014Article
- Matrilysin/matrix metalloproteinase-7(MMP7) cleavage of perlecan/HSPG2 creates a molecular switch to alter prostate cancer cell behavior.Matrix biology : journal of the International Society for Matrix Biology · 2014Article
- Border patrol: insights into the unique role of perlecan/heparan sulfate proteoglycan 2 at cell and tissue borders.Matrix biology : journal of the International Society for Matrix Biology · 2014Review
- Heparan sulfate in skeletal development, growth, and pathology: the case of hereditary multiple exostoses.Developmental dynamics : an official publication of the American Association of Anatomists · 2013Review
- Increased expression of heparanase in osteogenic differentiation of rat marrow stromal cells.Experimental and therapeutic medicine · 2013Article
- Extracellular matrix remodeling in experimental intervertebral disc degeneration.Acta ortopedica brasileira · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Endochondral bone formation is a highly orchestrated process involving coordination among cell-cell, cell-matrix and growth factor signaling that eventually results in the production of mineralized bone from a cartilage template. Chondrogenic and osteogenic differentiation occur in sequence during this process, and the temporospatial patterning clearly requires the activities of heparin binding growth factors and their receptors. Heparanase (HPSE) plays a role in osteogenesis, but the mechanism by which it does so is incompletely understood. We used a combination of ex vivo and in vitro approaches and a well described HPSE inhibitor, PI-88 to study HPSE in endochondral bone formation. In situ hybridization and immunolocalization with HPSE antibodies revealed that HPSE is expressed in the peri-chondrium, peri-osteum, and at the chondro-osseous junction, all sites of key signaling events and tissue morphogenesis. Transcripts encoding Hpse also were observed in the pre-hypertrophic zone. Addition of PI-88 to metatarsals in organ culture reduced growth and suggested that HPSE activity aids the transition from chondrogenic to osteogenic processes in growth of long bones. To study this, we used high density cultures of ATDC5 pre-chondrogenic cells grown under conditions favoring chondrogenesis or osteogenesis. Under chondrogenic conditions, HPSE/Hpse was expressed at high levels during the mid-culture period, at the onset of terminal chondrogenesis. PI-88 addition reduced chondrogenesis and accelerated osteogenesis, including a dramatic up-regulation of osteocalcin levels. In normal growth medium, addition of PI-88 reduced migration of ATDC-5 cells, suggesting that HPSE facilitates cartilage replacement by bone at the chondro-osseous junction by removing the HS component of proteoglycans, such as perlecan/HSPG2, that otherwise prevent osteogenic cells from remodeling hypertrophic cartilage.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.