ArticleBMC medical genomics2008
Characterization of global transcription profile of normal and HPV-immortalized keratinocytes and their response to TNF treatment.
Article in BMC medical genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- High Levels of Superoxide Dismutase 2 Are Associated With Worse Prognosis in Patients With Breast Cancer.European journal of breast health · 2024Article
- TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line.Scientific reports · 2022Article
- High Expression of SOD2 Protein Is a Strong Prognostic Factor for Stage IIIB Squamous Cell Cervical Carcinoma.Antioxidants (Basel, Switzerland) · 2021Article
- Tanshinone IIA inhibits glucose metabolism leading to apoptosis in cervical cancer.Oncology reports · 2019Article
- Innate immunity and HPV: friends or foes.Clinics (Sao Paulo, Brazil) · 2018Article
- Strong SOD2 expression and HPV-16/18 positivity are independent events in cervical cancer.Oncotarget · 2018Article
- The differential role of HTRA1 in HPV-positive and HPV-negative cervical cell line proliferation.BMC cancer · 2016Article
- The interferon-related developmental regulator 1 is used by human papillomavirus to suppress NFκB activation.Nature communications · 2015Article
- SOD2 immunoexpression predicts lymph node metastasis in penile cancer.BMC clinical pathology · 2015Article
- CD40-mediated amplification of local immunity by epithelial cells is impaired by HPV.The Journal of investigative dermatology · 2014Article
- Latexin sensitizes leukemogenic cells to gamma-irradiation-induced cell-cycle arrest and cell death through Rps3 pathway.Cell death & disease · 2014Article
- Article
- Latexin is down-regulated in hematopoietic malignancies and restoration of expression inhibits lymphoma growth.PloS one · 2012Article
- A Decade of Global mRNA and miRNA Profiling of HPV-Positive Cell Lines and Clinical Specimens.The open virology journal · 2012Article
- Gene network analyses point to the importance of human tissue kallikreins in melanoma progression.BMC medical genomics · 2011Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPersistent infection by high risk HPV types (e.g. HPV-16, -18, -31, and -45) is the main risk factor for development of cervical intraepithelial neoplasia and cervical cancer. Tumor necrosis factor (TNF) is a key mediator of epithelial cell inflammatory response and exerts a potent cytostatic effect on normal or HPV16, but not on HPV18 immortalized keratinocytes. Moreover, several cervical carcinoma-derived cell lines are resistant to TNF anti-proliferative effect suggesting that the acquisition of TNF-resistance may constitute an important step in HPV-mediated carcinogenesis. In the present study, we compared the gene expression profiles of normal and HPV16 or 18 immortalized human keratinocytes before and after treatment with TNF for 3 or 60 hours.
methodsIn this study, we determined the transcriptional changes 3 and 60 hours after TNF treatment of normal, HPV16 and HPV18 immortalized keratinocytes by microarray analysis. The expression pattern of two genes observed by microarray was confirmed by Northern Blot. NF-kappaB activation was also determined by electrophoretic mobility shift assay (EMSA) using specific oligonucleotides and nuclear protein extracts.
resultsWe observed the differential expression of a common set of genes in two TNF-sensitive cell lines that differs from those modulated in TNF-resistant ones. This information was used to define genes whose differential expression could be associated with the differential response to TNF, such as: KLK7 (kallikrein 7), SOD2 (superoxide dismutase 2), 100P (S100 calcium binding protein P), PI3 (protease inhibitor 3, skin-derived), CSTA (cystatin A), RARRES1 (retinoic acid receptor responder 1), and LXN (latexin). The differential expression of the KLK7 and SOD2 transcripts was confirmed by Northern blot. Moreover, we observed that SOD2 expression correlates with the differential NF-kappaB activation exhibited by TNF-sensitive and TNF-resistant cells.
conclusionThis is the first in depth analysis of the differential effect of TNF on normal and HPV16 or HPV18 immortalized keratinocytes. Our findings may be useful for the identification of genes involved in TNF resistance acquisition and candidate genes which deregulated expression may be associated with cervical disease establishment and/or progression.
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