ReviewNucleic acids research2008
See me, feel me: methods to concurrently visualize and manipulate single DNA molecules and associated proteins.
Review in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed.
- OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopy.Nucleic acids research · 2026Article
- Integrated In-Plane Nanofluidic Devices for Resistive-Pulse Sensing.Annual review of analytical chemistry (Palo Alto, Calif.) · 2024Review
- Optical Tweezers to Study Viruses.Sub-cellular biochemistry · 2024Review
- Optical traps induce fluorophore photobleaching by two-photon excitation.Biophysical journal · 2023Article
- Fabrication and microfluidic analysis of graphene-based molecular communication receiver for Internet of Nano Things (IoNT).Scientific reports · 2021Article
- Magnetic Tweezers-Based Single-Molecule Assays to Study Interaction of E. coli SSB with DNA and RecQ Helicase.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Optical tweezers in single-molecule biophysics.Nature reviews. Methods primers · 2021Article
- Single molecule mass photometry of nucleic acids.Nucleic acids research · 2020Article
- Co-temporal Force and Fluorescence Measurements Reveal a Ribosomal Gear Shift Mechanism of Translation Regulation by Structured mRNAs.Molecular cell · 2019Article
- NMR: an essential structural tool for integrative studies of T cell development, pMHC ligand recognition and TCR mechanobiology.Journal of biomolecular NMR · 2019Article
- Recent Developments in the Interactions of Classic Intercalated Ruthenium Compounds: [Ru(bpy)₂dppz]Molecules (Basel, Switzerland) · 2019Review
- Two distinct conformational states define the interaction of human RAD51-ATP with single-stranded DNA.The EMBO journal · 2018Article
- A change of view: homologous recombination at single-molecule resolution.Nature reviews. Genetics · 2018Review
- Article
- High-Resolution "Fleezers": Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Mechanisms of small molecule-DNA interactions probed by single-molecule force spectroscopy.Nucleic acids research · 2016Article
- A general approach to visualize protein binding and DNA conformation without protein labelling.Nature communications · 2016Article
- Observation of long-range tertiary interactions during ligand binding by the TPP riboswitch aptamer.eLife · 2015Article
- The fluorescence properties and binding mechanism of SYTOX green, a bright, low photo-damage DNA intercalating agent.European biophysics journal : EBJ · 2015Article
- A thiazole coumarin (TC) turn-on fluorescence probe for AT-base pair detection and multipurpose applications in different biological systems.Scientific reports · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct visualization of DNA and proteins allows researchers to investigate DNA-protein interactions with great detail. Much progress has been made in this area as a result of increasingly sensitive single-molecule fluorescence techniques. At the same time, methods that control the conformation of DNA molecules have been improving constantly. The combination of both techniques has appealed to researchers ever since single-molecule measurements have become possible and indeed first implementations of such combined approaches have proven useful in the study of several DNA-binding proteins in real time. Here, we describe the technical state-of-the-art of various integrated manipulation-and-visualization methods. We first discuss methods that allow only little control over the DNA conformation, such as DNA combing. We then describe DNA flow-stretching approaches that allow more control, and end with the full control on position and extension obtained by manipulating DNA with optical tweezers. The advantages and limitations of the various techniques are discussed, as well as several examples of applications to biophysical or biochemical questions. We conclude with an outlook describing potential future technical developments in combining fluorescence microscopy with DNA micromanipulation technology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.