ArticleJournal of molecular neuroscience : MN2009
Transcriptomic and proteomic analyses of mouse cerebellum reveals alterations in RasGRF1 expression following in vivo chronic treatment with delta 9-tetrahydrocannabinol.
Article in Journal of molecular neuroscience : MN, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
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- Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC).Proteomes · 2023Article
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- Effects of Rare Phytocannabinoids on the Endocannabinoid System of Human Keratinocytes.International journal of molecular sciences · 2022Article
- A Proteomic View of Cellular and Molecular Effects of Cannabis.Biomolecules · 2021Review
- Bisphenol A Deranges the Endocannabinoid System of Primary Sertoli Cells with an Impact on Inhibin B Production.International journal of molecular sciences · 2020Article
- LSD1 is an environmental stress-sensitive negative modulator of the glutamatergic synapse.Neurobiology of stress · 2020Article
- The Cerebellum, THC, and Cannabis Addiction: Findings from Animal and Human Studies.Cerebellum (London, England) · 2019Review
- Novel molecular changes induced by Nrg1 hypomorphism and Nrg1-cannabinoid interaction in adolescence: a hippocampal proteomic study in mice.Frontiers in cellular neuroscience · 2013Article
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- Endocannabinoids stimulate human melanogenesis via type-1 cannabinoid receptor.The Journal of biological chemistry · 2012Article
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- Genes and pathways co-associated with the exposure to multiple drugs of abuse, including alcohol, amphetamine/methamphetamine, cocaine, marijuana, morphine, and/or nicotine: a review of proteomics analyses.Molecular neurobiology · 2011Review
- Molecular model of cannabis sensitivity in developing neuronal circuits.Trends in pharmacological sciences · 2011Review
- Δ9-THC increases endogenous AHA1 expression in rat cerebellum and may modulate CB1 receptor function during chronic use.Journal of neurochemistry · 2011Article
- Lasting impacts of prenatal cannabis exposure and the role of endogenous cannabinoids in the developing brain.Future neurology · 2011Article
- Regulation of Neuronal Function by Ras-GRF Exchange Factors.Genes & cancer · 2011Article
- Characterization of the endocannabinoid system in human neuronal cells and proteomic analysis of anandamide-induced apoptosis.The Journal of biological chemistry · 2009Article
- Cannabinol modulates the endocannabinoid system and shows TRPV1-mediated anti-inflammatory properties in human keratinocytes.BioFactors (Oxford, England)Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have applied transcriptomic and proteomic techniques to identify changes in the RNA and the protein levels in the mouse cerebellum after chronic treatment with Delta(9)-tetrahydrocannabinol (THC). Among approximately 14,000 transcripts in a mouse cDNA microarray library, we found 11 genes with altered expression. RasGRF1, a neuron-specific Ras guanine nucleotide exchange factor, showed a reduction both at the RNA and protein levels with a specific decrease of the protein pool associated to cell membranes. In addition, proteomic analysis on cerebellum obtained from chronically THC-treated mice detected quantitative changes of additional 27 spots, mostly in the membranous fraction. We found enrichment of alpha (Galphao, Galphaq) and beta subunits (beta4/beta2 and beta5) of guanine nucleotide-binding proteins and of two calcium-binding proteins, calretinin and hippocalcin-like protein-1. In addition, we also detected a significant increase in the membrane fraction of proteins involved in exo-endocytosis such as septins, dynamin-1, and vesicle protein sorting 29. By western blotting, we confirmed increased membrane localization of calretinin and of dynamin-1 isoforms with higher isoelectric point, indicative for an underphosphorylated state of the molecule. In conclusion, our results indicate that chronic THC modulates the expression and subcellular localization of proteins implicated in Ras signaling, calcium-buffering potential, and trafficking.
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