ArticleMolecular biology reports2009
Spermatogenesis-related ring finger gene ZNF230 promoter: identification and functional analysis.
Article in Molecular biology reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Genome-wide identification and annotation of SNPs and their mapping in candidate genes related to milk production and fertility traits in Badri cattle.Tropical animal health and production · 2023Article
- Genome-wide discovery of SNPs in candidate genes related to production and fertility traits in Sahiwal cattle.Tropical animal health and production · 2020Article
- Expression profiles of Sox transcription factors within the postnatal rodent testes.Molecular and cellular biochemistry · 2018Article
- Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5.BMC molecular biology · 2017Article
- Replication analysis confirms the association of several variants with acute myeloid leukemia in Chinese population.Journal of cancer research and clinical oncology · 2016Article
- Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development.Molecular and cellular biochemistry · 2015Article
- Evaluation of five candidate genes from GWAS for association with oligozoospermia in a Han Chinese population.PloS one · 2013Article
- Haploinsufficiency of SOX5 at 12p12.1 is associated with developmental delays with prominent language delay, behavior problems, and mild dysmorphic features.Human mutation · 2012Article
- Transcriptional regulation of an axonemal central apparatus gene, sperm-associated antigen 6, by a SRY-related high mobility group transcription factor, S-SOX5.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ZNF230 gene is a recently cloned gene which is transcribed only in fertile male testes and may be related to human spermatogenesis. To characterize the multiple stage-specific transcription elements necessary for ZNF230 expression, we cloned ZNF230 promoter and constructed chimeric luciferase reporter Plasmids. Overexpression and site-directed mutation test were used to characterize the cis-element. The results showed ZNF230 gene promoter to be GC rich and not contain a TATA box. Deletion analysis of the 5'-flanking region of ZNF230 in HEK293 cells indicated that the sequence encompassing from nt -131 to +152 has a basal transcriptional activity. Site-directed mutation test and mithramycin A treatment demonstrated that the ZNF230 promoter contained a functional Sp1 site. Overexpression of the Sox5 protein activated the promoter activity. A 312-bp fragment surrounding the transcription start site exhibits a characteristic CpG island which overlaps with the promoter region. We also provided evidence that both the human and mouse znf230 promoter consist of Sp1 binding site and GC-rich sequences, suggesting Sp1 is required for the transcription of human and mouse ZNF230 genes. In conclusion, these findings suggest that ZNF230 is tightly controlled at transcriptional level and a common mechanism controls the basal transcription of ZNF230 gene.
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