ArticlePLoS genetics2008
Low levels of DNA polymerase alpha induce mitotic and meiotic instability in the ribosomal DNA gene cluster of Saccharomyces cerevisiae.
Article in PLoS genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- Global genomic instability caused by reduced expression of DNA polymerase ε in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Noncanonical outcomes of break-induced replication produce complex, extremely long-tract gene conversion events in yeast.G3 (Bethesda, Md.) · 2021Article
- Genome-wide mapping of spontaneous genetic alterations in diploid yeast cells.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The effects of manipulating levels of replication initiation factors on origin firing efficiency in yeast.PLoS genetics · 2019Article
- Review
- A Case Study of Genomic Instability in an Industrial Strain ofG3 (Bethesda, Md.) · 2018Article
- DNA replication stress restricts ribosomal DNA copy number.PLoS genetics · 2017Article
- Genome-destabilizing effects associated with top1 loss or accumulation of top1 cleavage complexes in yeast.PLoS genetics · 2015Article
- Essential role of the iron-sulfur cluster binding domain of the primase regulatory subunit Pri2 in DNA replication initiation.Protein & cell · 2015Article
- Genome-wide high-resolution mapping of chromosome fragile sites in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae.PLoS genetics · 2013Article
- Massive genomic variation and strong selection in Arabidopsis thaliana lines from Sweden.Nature genetics · 2013Article
- Fragile site instability in Saccharomyces cerevisiae causes loss of heterozygosity by mitotic crossovers and break-induced replication.PLoS genetics · 2013Article
- Haploidization in Saccharomyces cerevisiae induced by a deficiency in homologous recombination.Genetics · 2012Article
- Transgene-mediated cosuppression and RNA interference enhance germ-line apoptosis in Caenorhabditis elegans.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Replication stress-induced chromosome breakage is correlated with replication fork progression and is preceded by single-stranded DNA formation.G3 (Bethesda, Md.) · 2011Article
- Replicative age induces mitotic recombination in the ribosomal RNA gene cluster of Saccharomyces cerevisiae.PLoS genetics · 2011Article
- Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells.Journal of aging research · 2011Article
- DNA replication fidelity and cancer.Seminars in cancer biology · 2010Review
- Eco1 is important for DNA damage repair in S. cerevisiae.Cell cycle (Georgetown, Tex.) · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The ribosomal DNA (rDNA) genes of Saccharomyces cerevisiae are located in a tandem array of about 150 repeats. Using a diploid with markers flanking and within the rDNA array, we showed that low levels of DNA polymerase alpha elevate recombination between both homologues and sister chromatids, about five-fold in mitotic cells and 30-fold in meiotic cells. This stimulation is independent of Fob1p, a protein required for the programmed replication fork block (RFB) in the rDNA. We observed that the fob1 mutation alone significantly increased meiotic, but not mitotic, rDNA recombination, suggesting a meiosis-specific role for this protein. We found that meiotic cells with low polymerase alpha had decreased Sir2p binding and increased Spo11p-catalyzed double-strand DNA breaks in the rDNA. Furthermore, meiotic crossover interference in the rDNA is absent. These results suggest that the hyper-Rec phenotypes resulting from low levels of DNA polymerase alpha in mitosis and meiosis reflect two fundamentally different mechanisms: the increased mitotic recombination is likely due to increased double-strand DNA breaks (DSBs) resulting from Fob1p-independent stalled replication forks, whereas the hyper-Rec meiotic phenotype results from increased levels of Spo11-catalyzed DSBs in the rDNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.