ArticleThe EMBO journal2008
Molecular basis for maintenance of fidelity during the CCA-adding reaction by a CCA-adding enzyme.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Enzyme structure and kinetics produce tRNA and nucleotide specificity of Schizosaccharomyces pombe CC- and A-adding enzymes.Nucleic acids research · 2026Article
- Crystal structure of the CCA-adding enzyme fromJournal of structural biology: X · 2025Article
- Function and Regulation of Human Terminal Uridylyltransferases.Frontiers in genetics · 2018Review
- NMR reveals structural rearrangements associated to substrate insertion in nucleotide-adding enzymes.Protein science : a publication of the Protein Society · 2016Article
- Article
- Structures and functions of Qβ replicase: translation factors beyond protein synthesis.International journal of molecular sciences · 2014Review
- Molecular mechanisms of template-independent RNA polymerization by tRNA nucleotidyltransferases.Frontiers in genetics · 2014Review
- An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme.PLoS biology · 2013Article
- An inhibitory C-terminal region dictates the specificity of A-adding enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- How the CCA-adding enzyme selects adenine over cytosine at position 76 of tRNA.Science (New York, N.Y.) · 2010Article
- tRNA nucleotidyltransferases: ancient catalysts with an unusual mechanism of polymerization.Cellular and molecular life sciences : CMLS · 2010Review
- CCA addition to tRNA: implications for tRNA quality control.IUBMB life · 2010Review
- Transfer RNA processing in archaea: unusual pathways and enzymes.FEBS letters · 2010Review
- Mechanism for the definition of elongation and termination by the class II CCA-adding enzyme.The EMBO journal · 2009Article
- Distinct kinetic determinants for the stepwise CCA addition to tRNA.RNA (New York, N.Y.) · 2009Article
- Controlling translation via modulation of tRNA levels.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CCA-adding enzyme builds the 3'-end CCA of tRNA without a nucleic acid template. The mechanism for the maintenance of fidelity during the CCA-adding reaction remains elusive. Here, we present almost a dozen complex structures of the class I CCA-adding enzyme and tRNA mini-helices (mini-D(73)N(74), mini-D(73)N(74)C(75) and mini-D(73)C(74)N(75); D(73) is a discriminator nucleotide and N is either A, G, or U). The mini-D(73)N(74) complexes adopt catalytically inactive open forms, and CTP shifts the enzymes to the active closed forms and allows N(74) to flip for CMP incorporation. In contrast, unlike the catalytically active closed form of the mini-D(73)C(74)C(75) complex, the mini-D(73)N(74)C(75) and mini-D(73)C(74)N(75) complexes adopt inactive open forms. Only the mini-D(73)C(74)U(75) accepts AMP to a similar extent as mini-D(73)C(74)C(75), and ATP shifts the enzyme to a closed, active form and allows U(75) to flip for AMP incorporation. These findings suggest that the 3'-region of RNA is proofread, after two nucleotide additions, in the closed, active form of the complex at the AMP incorporation stage. This proofreading is a prerequisite for the maintenance of fidelity for complete CCA synthesis.
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