ArticlePlant physiology2008
Imaging of the Yellow Cameleon 3.6 indicator reveals that elevations in cytosolic Ca2+ follow oscillating increases in growth in root hairs of Arabidopsis.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
108 citing papers in PubMed, 1 synthesis or guideline pooled it, 248 citations in OpenAlex.
- Root hair growth from the pH point of view.Frontiers in plant science · 2022Pooled it
- MAcro Plant Projection Imaging (MAPPI): An open, scalable platform for whole-plant fluorescence real-time imaging.Science advances · 2026Article
- Arabidopsis IONIC CURRENT FAMILY A proteins facilitate environmental calcium acquisition essential for stress tolerance.Nature plants · 2026Article
- MLO-mediated CaThe New phytologist · 2025Article
- Response of Plants to Touch Stress at Morphological, Physiological and Molecular Levels.International journal of molecular sciences · 2025Review
- The C-terminal domain of SEC-10 is fundamental for exocyst function, apical organization, and cell morphogenesis inMolecular biology of the cell · 2025Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- CPK1 activates CNGCs through phosphorylation for CaNature communications · 2025Article
- Arabidopsis calcium-dependent protein kinases 4/5/6/11 negatively regulate hydrotropism via phosphorylation of MIZU-KUSSEI1.The Plant cell · 2024Article
- Understanding the mechanobiology of phytoacoustics through molecular Lens: Mechanisms and future perspectives.Journal of advanced research · 2024Review
- Article
- Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Calcium signaling in plant mineral nutrition: From uptake to transport.Plant communications · 2023Review
- The Developmental Mechanism of the Root System of Cultivated Terrestrial Watercress.Plants (Basel, Switzerland) · 2023Article
- Calcium Live Imaging at Multi-Scales from Cellular to Organ Level in Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2023Article
- ROS and calcium oscillations are required for polarized root hair growth.Plant signaling & behavior · 2022Review
- MCU proteins dominate in vivo mitochondrial Ca2+ uptake in Arabidopsis roots.The Plant cell · 2022Article
- Low Concentration of Aluminum-Stimulated Pollen Tube Growth of Apples (Plants (Basel, Switzerland) · 2022Article
- A novel calmodulin-interacting Domain of Unknown Function 506 protein represses root hair elongation in Arabidopsis.Plant, cell & environment · 2022Article
- Plant Bioelectronics and Biohybrids: The Growing Contribution of Organic Electronic and Carbon-Based Materials.Chemical reviews · 2022Review
48 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
In tip-growing cells, the tip-high Ca(2+) gradient is thought to regulate the activity of components of the growth machinery, including the cytoskeleton, Ca(2+)-dependent regulatory proteins, and the secretory apparatus. In pollen tubes, both the Ca(2+) gradient and cell elongation show oscillatory behavior, reinforcing the link between the two. We report that in growing root hairs of Arabidopsis (Arabidopsis thaliana), an oscillating tip-focused Ca(2+) gradient can be resolved through imaging of a cytosolically expressed Yellow Cameleon 3.6 fluorescence resonance energy transfer-based Ca(2+) sensor. Both elongation of the root hairs and the associated tip-focused Ca(2+) gradient show a similar dynamic character, oscillating with a frequency of 2 to 4 min(-1). Cross-correlation analysis indicates that the Ca(2+) oscillations lag the growth oscillations by 5.3 +/- 0.3 s. However, growth never completely stops, even during the slow cycle of an oscillation, and the concomitant tip Ca(2+) level is always slightly elevated compared with the resting Ca(2+) concentration along the distal shaft, behind the growing tip. Artificially increasing Ca(2+) using the Ca(2+) ionophore A23187 leads to immediate cessation of elongation and thickening of the apical cell wall. In contrast, dissipating the Ca(2+) gradient using either the Ca(2+) channel blocker La(3+) or the Ca(2+) chelator EGTA is accompanied by an increase in the rate of cell expansion and eventual bursting of the root hair tip. These observations are consistent with a model in which the maximal oscillatory increase in cytosolic Ca(2+) is triggered by cell expansion associated with tip growth and plays a role in the subsequent restriction of growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.