ArticlePlant physiology2008
Bridging the gap between plant and mammalian polyamine catabolism: a novel peroxisomal polyamine oxidase responsible for a full back-conversion pathway in Arabidopsis.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 199 citations in OpenAlex.
- Gamma-aminobutyric acid (GABA) releases seed dormancy by orchestrating abscisic acid and gibberellin metabolism and signaling.BMC plant biology · 2025Article
- Polyamine oxidation enzymes regulate sexual mating/filamentation and pathogenicity in Sporisorium scitamineum.Molecular plant pathology · 2024Article
- Molecular Insights into the Synergistic Effects of Putrescine and Ammonium on Dinoflagellates.International journal of molecular sciences · 2024Article
- Polyamine-Derived Aminoaldehydes and Acrolein: Cytotoxicity, Reactivity and Analysis of the Induced Protein Modifications.Molecules (Basel, Switzerland) · 2023Review
- Interactions of Polyamines and Phytohormones in Plant Response to Abiotic Stress.Plants (Basel, Switzerland) · 2023Review
- CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion.International journal of molecular sciences · 2022Article
- Genome-Wide Identification of Polyamine Oxidase (PAO) Family Genes: Roles ofInternational journal of molecular sciences · 2022Article
- Polyamine Oxidase Triggers HInternational journal of molecular sciences · 2022Article
- Responses of Polyamine-Metabolic Genes to Polyamines and Plant Stress Hormones in Arabidopsis Seedlings.Cells · 2021Article
- Effect of thermospermine on expression profiling of different gene using massive analysis of cDNA ends (MACE) and vascular maintenance in Arabidopsis.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Article
- Comparative Genomic Analysis of Quantitative Trait Loci Associated With Micronutrient Contents, Grain Quality, and Agronomic Traits in Wheat (Frontiers in plant science · 2021Article
- The tree of life of polyamine oxidases.Scientific reports · 2020Article
- uORFs: ImportantInternational journal of molecular sciences · 2020Review
- Article
- Article
- Genome-Wide Identification of Seven Polyamine Oxidase Genes inFrontiers in plant science · 2020Article
- Plant Peroxisomes: A Factory of Reactive Species.Frontiers in plant science · 2020Review
- Genetically Modified Heat Shock Protein90s and Polyamine Oxidases inPlants (Basel, Switzerland) · 2019Article
- The Interplay among Polyamines and Nitrogen in Plant Stress Responses.Plants (Basel, Switzerland) · 2019Review
15 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to animals, where polyamine (PA) catabolism efficiently converts spermine (Spm) to putrescine (Put), plants have been considered to possess a PA catabolic pathway producing 1,3-diaminopropane, Delta(1)-pyrroline, the corresponding aldehyde, and hydrogen peroxide but unable to back-convert Spm to Put. Arabidopsis (Arabidopsis thaliana) genome contains at least five putative PA oxidase (PAO) members with yet-unknown localization and physiological role(s). AtPAO1 was recently identified as an enzyme similar to the mammalian Spm oxidase, which converts Spm to spermidine (Spd). In this work, we have performed in silico analysis of the five Arabidopsis genes and have identified PAO3 (AtPAO3) as a nontypical PAO, in terms of homology, compared to other known PAOs. We have expressed the gene AtPAO3 and have purified a protein corresponding to it using the inducible heterologous expression system of Escherichia coli. AtPAO3 catalyzed the sequential conversion/oxidation of Spm to Spd, and of Spd to Put, thus exhibiting functional homology to the mammalian PAOs. The best substrate for this pathway was Spd, whereas the N(1)-acetyl-derivatives of Spm and Spd were oxidized less efficiently. On the other hand, no activity was detected when diamines (agmatine, cadaverine, and Put) were used as substrates. Moreover, although AtPAO3 does not exhibit significant similarity to the other known PAOs, it is efficiently inhibited by guazatine, a potent PAO inhibitor. AtPAO3 contains a peroxisomal targeting motif at the C terminus, and it targets green fluorescence protein to peroxisomes when fused at the N terminus but not at the C terminus. These results reveal that AtPAO3 is a peroxisomal protein and that the C terminus of the protein contains the sorting information. The overall data reinforce the view that plants and mammals possess a similar PA oxidation system, concerning both the subcellular localization and the mode of its action.
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