ArticleMolecular and cellular endocrinology2008
Ecdysone [corrected] receptor isoforms play distinct roles in controlling molting and metamorphosis in the red flour beetle, Tribolium castaneum.
Article in Molecular and cellular endocrinology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 123 citations in OpenAlex.
- RNA interference knockdown of the nuclear receptor HR3 suppresses vitellogenesis in Chilo suppressalis.Molecular biology reports · 2025Article
- Role of histone methylation in insect development: KMT5A regulates ecdysteroid biosynthesis during metamorphosis of Tribolium castaneum.Insect biochemistry and molecular biology · 2025Article
- Azadirachtin disrupts ecdysone signaling and alters sand fly immunity.Parasites & vectors · 2024Article
- N(alpha)-acetyltransferase 40-mediated histone acetylation plays an important role in ecdysone regulation of metamorphosis in the red flour beetle, Tribolium castaneum.Communications biology · 2024Article
- TGF-β Type II Receptor Punt Suppresses Antimicrobial Peptide Expression and Influences Development inInsects · 2023Article
- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
- The TGF-β Receptor GeneMolecules (Basel, Switzerland) · 2022Article
- The uncommon function and mechanism of the common enzyme glyceraldehyde-3-phosphate dehydrogenase in the metamorphosis ofFrontiers in bioengineering and biotechnology · 2022Article
- Neuropeptide Bursicon Influences Reproductive Physiology inFrontiers in physiology · 2021Article
- Ecdysone receptor isoforms play distinct roles in larval-pupal-adult transition in Leptinotarsa decemlineata.Insect science · 2020Article
- Post-transcriptional regulation of insect metamorphosis and oogenesis.Cellular and molecular life sciences : CMLS · 2020Review
- Ovarian Transcriptomic Analyses in the Urban Human Health Pest, the Western Black Widow Spider.Genes · 2020Article
- Identification of Halloween Genes and RNA Interference-Mediated Functional Characterization of a Halloween GeneFrontiers in physiology · 2019Article
- A Crucial Caste Regulation Gene Detected by Comparing Termites and Sister Group Cockroaches.Genetics · 2018Article
- Structure and function of the alternatively spliced isoforms of the ecdysone receptor gene in the Chinese mitten crab, Eriocheir sinensis.Scientific reports · 2017Article
- The effect of silencing 20E biosynthesis relative genes by feeding bacterially expressed dsRNA on the larval development of Chilo suppressalis.Scientific reports · 2016Article
- Molecular cloning, characterization, and expression analysis of an ecdysone receptor homolog in Teleogryllus emma (Orthoptera: Gryllidae).Journal of insect science (Online) · 2015Article
- Endocrine regulation of predator-induced phenotypic plasticity.Oecologia · 2014Article
- The POU factor ventral veins lacking/Drifter directs the timing of metamorphosis through ecdysteroid and juvenile hormone signaling.PLoS genetics · 2014Article
- Transcriptome-based identification of ABC transporters in the western tarnished plant bug Lygus hesperus.PloS one · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Ecdysteroids regulate insect growth and development through a heterodimeric complex of nuclear receptors consisting of ecdysone receptor (EcR) and ultraspiracle (USP). In the red flour beetle, Tribolium castaneum, two isoforms each of EcR and USP have been identified. Quantitative real-time reverse-transcriptase PCR (qRT-PCR) analysis showed isoform-specific developmental expression of both EcR and USP in the epidermis and the midgut dissected from the final instar larvae and pupae. Injection of double-stranded RNA (dsRNA) prepared using the common or isoform-specific regions of EcR or USP as templates caused derailment of development. EcR common region (EcRC) or EcRA dsRNA caused more severe effects, and most of the treated larvae died prior to pupation. EcRB dsRNA caused less severe effects and most of the treated larvae became pupae but showed developmental defects. Only dsRNA prepared against USP common region but not against USPA or USPB isoform-specific region caused developmental defects during larval-pupal metamorphosis. Determination of mRNA levels of EcR isoforms and 20-hydroxyecdysone-response (20E) genes (broad, E75, E74, HR3 and FTZ-F1) by qRT-PCR in the larvae injected with EcRA, EcRB or EcRC dsRNA showed that EcRA initiates ecdysteroid action by regulation the expression of EcRB and 20E-response genes. These data suggest that the EcR but not USP isoforms play distinct roles during the larval-pupal metamorphosis and EcRA plays a dominant role in transduction of ecdysteroid response in T. castaneum.
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