ArticleJournal of molecular biology2008
Asymmetric nucleotide transactions of the HslUV protease.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Ancestral [Fe-S] biogenesis system SMS has a unique mechanism of cluster assembly and sulfur utilization.PLoS biology · 2025Article
- Protein degradation control and regulation of bacterial survival and pathogenicity: the role of protein degradation systems in bacteria.Molecular biology reports · 2021Review
- Article
- Proteolysis mediated by the membrane-integrated ATP-dependent protease FtsH has a unique nonlinear dependence on ATP hydrolysis rates.Protein science : a publication of the Protein Society · 2019Article
- The HslV Protease fromInternational journal of molecular sciences · 2019Article
- Structure and Function of the 26S Proteasome.Annual review of biochemistry · 2018Review
- Folding-Degradation Relationship of a Membrane Protein Mediated by the Universally Conserved ATP-Dependent Protease FtsH.Journal of the American Chemical Society · 2018Article
- The AAA ATPase Vps4 binds ESCRT-III substrates through a repeating array of dipeptide-binding pockets.eLife · 2017Article
- Structure of the mitochondrial inner membrane AAA+ protease YME1 gives insight into substrate processing.Science (New York, N.Y.) · 2017Article
- Covalently linked HslU hexamers support a probabilistic mechanism that links ATP hydrolysis to protein unfolding and translocation.The Journal of biological chemistry · 2017Article
- High-resolution cryo-EM structure of the proteasome in complex with ADP-AlFx.Cell research · 2017Article
- Cryo-EM structures of the eukaryotic replicative helicase bound to a translocation substrate.Nature communications · 2016Article
- Coarse-Grained Simulations of Topology-Dependent Mechanisms of Protein Unfolding and Translocation Mediated by ClpY ATPase Nanomachines.PLoS computational biology · 2016Article
- The Pex1/Pex6 complex is a heterohexameric AAA+ motor with alternating and highly coordinated subunits.Journal of molecular biology · 2015Article
- Cooperation of Hsp70 and Hsp100 chaperone machines in protein disaggregation.Frontiers in molecular biosciences · 2015Review
- Article
- Regulated protein turnover: snapshots of the proteasome in action.Nature reviews. Molecular cell biology · 2014Review
- Marching to the beat of the ring: polypeptide translocation by AAA+ proteases.Trends in biochemical sciences · 2014Review
- Insights into the molecular evolution of HslU ATPase through biochemical and mutational analyses.PloS one · 2014Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
ATP binding and hydrolysis are critical for protein degradation by HslUV, a AAA(+) machine containing one or two HslU(6) ATPases and the HslV(12) peptidase. Although each HslU homohexamer has six potential ATP-binding sites, we show that only three or four ATP molecules bind at saturation and present evidence for three functional subunit classes. These results imply that only a subset of HslU and HslUV crystal structures represents functional enzyme conformations. Our results support an asymmetric mechanism of ATP binding and hydrolysis, and suggest that molecular contacts between HslU and HslV vary dynamically throughout the ATPase cycle. Nucleotide binding controls HslUV assembly and activity. Binding of a single ATP allows HslU to bind HslV, whereas additional ATPs must bind HslU to support substrate recognition and to activate ATP hydrolysis, which powers substrate unfolding and translocation. Thus, a simple thermodynamic hierarchy ensures that substrates bind to functional HslUV complexes, that ATP hydrolysis is efficiently coupled to protein degradation, and that working HslUV does not dissociate, allowing highly processive degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.