ArticleJournal of clinical microbiology2008
Single-step PCR using (GACA)4 primer: utility for rapid identification of dermatophyte species and strains.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Comparative Description of Dermatophyte Genomes: A State-of-the-Art Review.Mycopathologia · 2023Pooled it
- Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia.Biomedica : revista del Instituto Nacional de Salud · 2022Article
- Identification of Fungi Involved in Onychomycosis in Patients of a Spanish Rural Area.Journal of fungi (Basel, Switzerland) · 2021Article
- Identification and characterization of dermatophyte species and strains with PCR amplification.Experimental and therapeutic medicine · 2014Article
- Microsatellite-primed PCR for intra-species genetic relatedness in Trichophyton ajelloi strains isolated in poland from various soil samples.Microbes and environments · 2014Article
- Morphological and molecular identification of two strains of dermatophytes.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2013Article
- Microsatellite-primed PCR and random primer amplification polymorphic DNA for the identification and epidemiology of dermatophytes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Use of Single-enzyme PCR-restriction Digestion Barcode Targeting the Internal Transcribed Spacers (ITS rDNA) to Identify Dermatophyte Species.Iranian journal of public health · 2012Article
- Characterization of the oral fungal microbiome (mycobiome) in healthy individuals.PLoS pathogens · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dermatophytes are fungi that belong to three genera: Epidermophyton, Microsporum, and Trichophyton. Identification of dermatophyte species is essential for appropriate diagnosis and treatment of dermatophytosis. Routine identification depends on macroscopic and microscopic morphology, which is time-consuming and does not identify dermatophyte strains. In this study, two PCR-based methods were compared for their abilities to identify 21 dermatophyte isolates obtained from Egyptian patients to the species and strain levels. The first method employed a two-step method: PCR amplification, using ITS1 and ITS4 as primers, followed by restriction enzyme digestion using the endonuclease MvaI. The second method employed a one-step approach employing the repetitive oligonucleotide (GACA)(4) as a primer. Dermatophyte strains were also identified using a conventional culture method. Our results showed that the conventional culture method identified four species: Microsporum canis, Trichophyton mentagrophytes, Trichophyton rubrum, and Trichophyton violaceum. Moreover, both PCR methods agreed with the diagnosis made using the conventional approach. Furthermore, ITS1/ITS4-based PCR provided no strain differentiation, while (GACA)(4)-based PCR identified different varieties among the T. mentagrophytes isolates. Taken together, our results suggest that (GACA)(4)-based PCR has utility as a simple and rapid method for identification of dermatophyte species as well as utility for differentiation of T. mentagrophytes variants.
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