ArticleThe Journal of biological chemistry2008
The talin rod IBS2 alpha-helix interacts with the beta3 integrin cytoplasmic tail membrane-proximal helix by establishing charge complementary salt bridges.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- α-Actinin-1 in Megakaryocytes: Its Structure, Interacting Proteins and Implications for Thrombopoiesis.Biomedicines · 2025Review
- Integrin Regulators in Neutrophils.Cells · 2022Review
- Review
- Targeting the RT loop of Src SH3 in Platelets Prevents Thrombosis without Compromising Hemostasis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Tetrapeptide Ac-HAEE-NHInternational journal of molecular sciences · 2020Article
- Silk fibroin hydrogel promote burn wound healing through regulating TLN1 expression and affecting cell adhesion and migration.Journal of materials science. Materials in medicine · 2020Article
- Rare Missense Variants in TLN1 Are Associated With Familial and Sporadic Spontaneous Coronary Artery Dissection.Circulation. Genomic and precision medicine · 2019Article
- Pulmonary Endothelial Mechanical Sensing and Signaling, a Story of Focal Adhesions and Integrins in Ventilator Induced Lung Injury.Frontiers in physiology · 2019Review
- The tale of two talins - two isoforms to fine-tune integrin signalling.FEBS letters · 2018Review
- Review
- Article
- Alternative mechanisms for talin to mediate integrin function.Current biology : CB · 2015Article
- Leukocyte arrest: Biomechanics and molecular mechanisms of β2 integrin activation.Biorheology · 2015Review
- Talin regulates moesin-NHE-1 recruitment to invadopodia and promotes mammary tumor metastasis.The Journal of cell biology · 2014Article
- Atomic basis for the species-specific inhibition of αV integrins by monoclonal antibody 17E6 is revealed by the crystal structure of αVβ3 ectodomain-17E6 Fab complex.The Journal of biological chemistry · 2014Article
- Mechanisms of talin-dependent integrin signaling and crosstalk.Biochimica et biophysica acta · 2014Review
- Talins and kindlins: partners in integrin-mediated adhesion.Nature reviews. Molecular cell biology · 2013Review
- The unfolded von Willebrand factor response in bloodstream: the self-association perspective.Journal of hematology & oncology · 2012Review
- The C terminus of talin links integrins to cell cycle progression.The Journal of cell biology · 2011Article
- Endothelial cell talin1 is essential for embryonic angiogenesis.Developmental biology · 2011Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Talin establishes a major link between integrins and actin filaments and contains two distinct integrin binding sites: one, IBS1, located in the talin head domain and involved in integrin activation and a second, IBS2, that maps to helix 50 of the talin rod domain and is essential for linking integrin beta subunits to the cytoskeleton ( Moes, M., Rodius, S., Coleman, S. J., Monkley, S. J., Goormaghtigh, E., Tremuth, L., Kox, C., van der Holst, P. P., Critchley, D. R., and Kieffer, N. (2007) J. Biol. Chem. 282, 17280-17288 ). Through the combined approach of mutational analysis of the beta3 integrin cytoplasmic tail and the talin rod IBS2 site, SPR binding studies, as well as site-specific antibody inhibition experiments, we provide evidence that the integrin beta3-talin rod interaction relies on a helix-helix association between alpha-helix 50 of the talin rod domain and the membrane-proximal alpha-helix of the beta3 integrin cytoplasmic tail. Moreover, charge complementarity between the highly conserved talin rod IBS2 lysine residues and integrin beta3 glutamic acid residues is necessary for this interaction. Our results support a model in which talin IBS2 binds to the same face of the beta3 subunit cytoplasmic helix as the integrin alphaIIb cytoplasmic tail helix, suggesting that IBS2 can only interact with the beta3 subunit following integrin activation.
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