ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2008
Myotube formation on micro-patterned glass: intracellular organization and protein distribution in C2C12 skeletal muscle cells.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Extracellular-matrix mediated stresses spatially bias myoblast fusion and myotube growth.Nature communications · 2026Article
- Scaling Cultured Meat: Challenges and Solutions for Affordable Mass Production.Comprehensive reviews in food science and food safety · 2025Review
- Impact of Uniaxial Static Strain on Myoblast Differentiation in Collagen-Coated PCL Microfilament Scaffolds: Role of Onset Time of Mechanical Stimulation.Bioengineering (Basel, Switzerland) · 2024Article
- Effect of mTORC Agonism via MHY1485 with and without Rapamycin on C2C12 Myotube Metabolism.International journal of molecular sciences · 2024Article
- Optimization of H9c2 differentiation leads to calcium-active and striated cardiac cells without addition of retinoic acid.Frontiers in cell and developmental biology · 2024Article
- 3D Co-Printing and Substrate Geometry Influence the Differentiation of C2C12 Skeletal Myoblasts.Gels (Basel, Switzerland) · 2023Article
- Global Quantitative Proteomics Analysis Reveals the Downstream Signaling Networks of Msx1 and Msx2 in Myoblast Differentiation.Phenomics (Cham, Switzerland) · 2022Article
- Myogenic Potential of Extracellular Matrix Derived from Decellularized Bovine Pericardium.International journal of molecular sciences · 2021Article
- Directional topography gradients drive optimum alignment and differentiation of human myoblasts.Journal of tissue engineering and regenerative medicine · 2019Article
- (-)-Epicatechin stimulates mitochondrial biogenesis and cell growth in C2C12 myotubes via the G-protein coupled estrogen receptor.European journal of pharmacology · 2018Article
- Non-equivalence of nuclear import among nuclei in multinucleated skeletal muscle cells.Journal of cell science · 2018Article
- Ternary Aligned Nanofibers of RGD Peptide-Displaying M13 Bacteriophage/PLGA/Graphene Oxide for Facilitated Myogenesis.Nanotheranostics · 2018Article
- Study of myoblast differentiation using multi-dimensional scaffolds consisting of nano and micropatterns.Biomaterials research · 2017Article
- Anisotropic Materials for Skeletal-Muscle-Tissue Engineering.Advanced materials (Deerfield Beach, Fla.) · 2016Review
- Homologous Transcription Factors DUX4 and DUX4c Associate with Cytoplasmic Proteins during Muscle Differentiation.PloS one · 2016Article
- Insulin-Based Regulation of Glucose-functionalized Nanoparticle Uptake in Muscle Cells.Journal of materials chemistry. B · 2014Article
- Injectable skeletal muscle matrix hydrogel promotes neovascularization and muscle cell infiltration in a hindlimb ischemia model.European cells & materials · 2012Article
- An image analysis method for the precise selection and quantitation of fluorescently labeled cellular constituents: application to the measurement of human muscle cells in culture.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Article
- In vitro expression profiling of myostatin, follistatin, decorin and muscle-specific transcription factors in adult caprine contractile myotubes.Journal of muscle research and cell motility · 2011Article
- Controlling the orientation and synaptic differentiation of myotubes with micropatterned substrates.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proliferation and fusion of myoblasts are needed for the generation and repair of multinucleated skeletal muscle fibers in vivo. Studies of myocyte differentiation, cell fusion, and muscle repair are limited by an appropriate in vitro muscle cell culture system. We developed a novel cell culture technique [two-dimensional muscle syncytia (2DMS) technique] that results in formation of myotubes, organized in parallel much like the arrangement in muscle tissue. This technique is based on UV lithography-produced micro-patterned glass on which conventionally cultured C2C12 myoblasts proliferate, align, and fuse to neatly arranged contractile myotubes in parallel arrays. Combining this technique with fluorescent microscopy, we observed alignment of actin filament bundles and a perinuclear distribution of glucose transporter 4 after myotube formation. Newly formed myotubes contained adjacently located MyoD-positive and MyoD-negative nuclei, suggesting fusion of MyoD-positive and MyoD-negative cells. In comparison, the closely related myogenic factor Myf5 did not exhibit this pattern of distribution. Furthermore, cytoplasmic patches of MyoD colocalized with bundles of filamentous actin near myotube nuclei. At later stages of differentiation, all nuclei in the myotubes were MyoD negative. The 2DMS system is thus a useful tool for studies on muscle alignment, differentiation, fusion, and subcellular protein localization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.