ArticleThe Journal of biological chemistry2008
A flexible template boundary element in the RNA subunit of fission yeast telomerase.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- A high-quality reference genome for the fission yeast Schizosaccharomyces osmophilus.G3 (Bethesda, Md.) · 2023Article
- Telomeres and Telomere Length: A General Overview.Cancers · 2020Review
- Structural biology of telomeres and telomerase.Cellular and molecular life sciences : CMLS · 2020Review
- New perspectives on telomerase RNA structure and function.Wiley interdisciplinary reviews. RNA · 2018Review
- TeloPCR-seq: a high-throughput sequencing approach for telomeres.FEBS letters · 2016Article
- The functional requirement of two structural domains within telomerase RNA emerged early in eukaryotes.Nucleic acids research · 2016Article
- Telomerase RNA is more than a DNA template.RNA biology · 2016Review
- Evolutionary perspectives of telomerase RNA structure and function.RNA biology · 2016Review
- Identification of novel noncoding transcripts in telomerase-negative yeast using RNA-seq.Scientific reports · 2016Article
- Physical Connectivity Mapping by Circular Permutation of Human Telomerase RNA Reveals New Regions Critical for Activity and Processivity.Molecular and cellular biology · 2016Article
- Protein-RNA interaction restricts telomerase from running through the stop sign.Nature structural & molecular biology · 2015Article
- Telomerase RNA stem terminus element affects template boundary element function, telomere sequence, and shelterin binding.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Diverse mechanisms for spliceosome-mediated 3' end processing of telomerase RNA.Nature communications · 2015Article
- Telomere les(i/s)ons from a telomerase RNA mutant.Cell cycle (Georgetown, Tex.) · 2015Review
- RNA connectivity requirements between conserved elements in the core of the yeast telomerase RNP.The EMBO journal · 2013Article
- Specific features of telomerase RNA from Hansenula polymorpha.RNA (New York, N.Y.) · 2013Article
- Finding the end: recruitment of telomerase to telomeres.Nature reviews. Molecular cell biology · 2013Review
- The common ancestral core of vertebrate and fungal telomerase RNAs.Nucleic acids research · 2013Article
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Telomerase adds telomeric repeat sequences to chromosome ends using a short region of its RNA subunit as a template. Telomerase RNA subunits are phylogenetically highly divergent, and different strategies have evolved to demarcate the boundary of the template region. The recent identification of the gene encoding telomerase RNA in the fission yeast Schizosaccharomyces pombe (ter1+) has opened the door for structure-function analyses in a model that shares many features with the telomere maintenance machinery of higher eukaryotes. Here we describe a structural element in TER1 that defines the 5' boundary of the template. Disruption of a predicted long range base pairing interaction between template-adjacent nucleotides and a sequence further upstream resulted in reverse transcription beyond the template region and caused telomere shortening. Normal telomere length was restored by combining complementary nucleotide substitutions in both elements, showing that base pairing, not a specific sequence, limits reverse transcription beyond the template. The template boundary described here resembles that of budding yeasts and some mammalian telomerases. However, unlike any previously characterized boundary element, part of the paired region overlaps with the template itself, thus necessitating disruption of these interactions during most reverse transcription cycles. We show that changes in the paired region directly affect the length of individual telomeric repeat units. Our data further illustrate that marginal alignment of the telomeric 3' end with RNA sequences downstream of the template is responsible for primer slippage, causing incorporation of strings of guanosines at the start of a subset of repeats.
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