ArticleMolecular and cellular biology2008
In Xenopus egg extracts, DNA replication initiates preferentially at or near asymmetric AT sequences.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Stranded short nascent strand sequencing reveals the topology of DNA replication origins ineLife · 2026Article
- SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.Nature communications · 2020Article
- Single-molecule analysis reveals that DNA replication dynamics vary across the course of schizogony in the malaria parasite Plasmodium falciparum.Scientific reports · 2017Article
- Article
- Article
- Genome Duplication: The Heartbeat of Developing Organisms.Current topics in developmental biology · 2016Review
- Article
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- Back to the origin: reconsidering replication, transcription, epigenetics, and cell cycle control.Genes & cancer · 2012Article
- The chromatin backdrop of DNA replication: lessons from genetics and genome-scale analyses.Biochimica et biophysica acta · 2012Review
- Single-molecule analysis of DNA replication in Xenopus egg extracts.Methods (San Diego, Calif.) · 2012Review
- Polo-like kinase 1 (Plk1): an Unexpected Player in DNA Replication.Cell division · 2012Article
- Atomic scissors: a new method of tracking the 5-bromo-2'-deoxyuridine-labeled DNA in situ.PloS one · 2012Article
- Most anti-BrdU antibodies react with 2'-deoxy-5-ethynyluridine -- the method for the effective suppression of this cross-reactivity.PloS one · 2012Article
- Novel features of ARS selection in budding yeast Lachancea kluyveri.BMC genomics · 2011Article
- A distinct first replication cycle of DNA introduced in mammalian cells.Nucleic acids research · 2011Article
- Regulation of DNA replication by chromatin structures: accessibility and recruitment.Chromosoma · 2011Review
- Eukaryotic DNA replication origins: many choices for appropriate answers.Nature reviews. Molecular cell biology · 2010Review
- Arabidopsis thaliana chromosome 4 replicates in two phases that correlate with chromatin state.PLoS genetics · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous observations led to the conclusion that in Xenopus eggs and during early development, DNA replication initiates at regular intervals but with no apparent sequence specificity. Conversely, here, we present evidence for site-specific DNA replication origins in Xenopus egg extracts. Using lambda DNA, we show that DNA replication origins are activated in clusters in regions that contain closely spaced adenine or thymine asymmetric tracks used as preferential initiation sites. In agreement with these data, AT-rich asymmetric sequences added as competitors preferentially recruit origin recognition complexes and inhibit sperm chromatin replication by increasing interorigin spacing. We also show that the assembly of a transcription complex favors origin activity at the corresponding site without necessarily eliminating the other origins. Thus, although Xenopus eggs have the ability to replicate any kind of DNA, AT-rich domains or transcription factors favor the selection of DNA replication origins without increasing the overall efficiency of DNA synthesis. These results suggest that asymmetric AT-rich regions might be default elements that favor the selection of a DNA replication origin in a transcriptionally silent complex, whereas other epigenetic elements linked to the organization of domains for transcription may have further evolved over this basal layer of regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.