ArticleProtein science : a publication of the Protein Society2008
Engineering of halophilic enzymes: two acidic amino acid residues at the carboxy-terminal region confer halophilic characteristics to Halomonas and Pseudomonas nucleoside diphosphate kinases.
Article in Protein science : a publication of the Protein Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- Mechanistic insights into redox activity and catalytic determinants of the haloarchaeal flavin-dependent oxidoreductasebioRxiv : the preprint server for biology · 2026Article
- Microbial diversity in polyextreme salt flats and their potential applications.Environmental science and pollution research international · 2024Review
- Properties and Applications of Extremozymes from Deep-Sea Extremophilic Microorganisms: A Mini Review.Marine drugs · 2019Review
- Structural and Mechanistic Insights into the Improvement of the Halotolerance of a Marine Microbial Esterase by Increasing Intra- and Interdomain Hydrophobic Interactions.Applied and environmental microbiology · 2017Article
- Multifactorial level of extremostability of proteins: can they be exploited for protein engineering?Extremophiles : life under extreme conditions · 2017Review
- Structural and functional insights into thermally stable cytochrome c' from a thermophile.Protein science : a publication of the Protein Society · 2017Article
- Deciphering the Translation Initiation Factor 5A Modification Pathway in Halophilic Archaea.Archaea (Vancouver, B.C.) · 2016Article
- Cloning, expression and characterization of a novel cold‑adapted GDSL family esterase from Photobacterium sp. strain J15.Extremophiles : life under extreme conditions · 2016Article
- Rational engineering of a mesohalophilic carbonic anhydrase to an extreme halotolerant biocatalyst.Nature communications · 2015Article
- Nucleoside Diphosphate Kinase from Psychrophilic Pseudoalteromonas sp. AS-131 Isolated from Antarctic Ocean.The protein journal · 2015Article
- Review
- Structure of nucleoside diphosphate kinase from pacific shrimp (Litopenaeus vannamei) in binary complexes with purine and pyrimidine nucleoside diphosphates.Acta crystallographica. Section F, Structural biology communications · 2014Article
- A novel mercuric reductase from the unique deep brine environment of Atlantis II in the Red Sea.The Journal of biological chemistry · 2014Article
- Protective role of salt in catalysis and maintaining structure of halophilic proteins against denaturation.Frontiers in microbiology · 2014Review
- Protein hypersaline adaptation: insight from amino acids with machine learning algorithms.The protein journal · 2013Article
- Protein adaptations in archaeal extremophiles.Archaea (Vancouver, B.C.) · 2013Review
- Overexpression in a non-native halophilic host and biotechnological potential of NAD+-dependent glutamate dehydrogenase from Halobacterium salinarum strain NRC-36014.Extremophiles : life under extreme conditions · 2012Article
- A structural mechanism for dimeric to tetrameric oligomer conversion in Halomonas sp. nucleoside diphosphate kinase.Protein science : a publication of the Protein Society · 2012Article
- Distinct characteristics of single starch-binding domain SBD1 derived from tandem domains SBD1-SBD2 of halophilic Kocuria varians alpha-amylase.The protein journal · 2012Article
- Halophilic properties of metal binding protein characterized by high histidine content from Chromohalobacter salexigens DSM3043.The protein journal · 2012Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Nucleoside diphosphate kinase from Halomonas sp. 593 (HaNDK) exhibits halophilic characteristics. Residues 134 and 135 in the carboxy-terminal region of HaNDK are Glu-Glu, while those of its homologous counterpart of non-halophilic Pseudomonas NDK (PaNDK) are Ala-Ala. The double mutation, E134A-E135A, in HaNDK results in the loss of the halophilic characteristics, and, conversely, the double mutation of A134E-A135E in PaNDK confers halophilic characters to this enzyme, indicating that the charged state of these two residues that are located in the C-terminal region plays a critical role in determining halophilic characteristics. The importance of these two residues versus the net negative charges will be discussed in relation to the halophilicity of NDK.
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