Evidence map›Paper›PMID 18573555›Full record

ArticleJournal of microbiological methods2008

Skim milk enhances the preservation of thawed -80 degrees C bacterial stocks.

William L Cody, James W Wilson, David R Hendrixson, Kevin S McIver, Kayla E Hagman, C M Ott, Cheryl A Nickerson, Michael J Schurr

Abstract readEvaluation Study
In one paragraph

Article in Journal of microbiological methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Genetic Characterization of Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2026
    Article
  3. Article
  4. International journal of systematic and evolutionary microbiology · 2024
    Article
  5. Article
  6. Article
  7. Prevalence of Carbapenemases in Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2023
    Article
  8. Article
  9. Frontiers in microbiology · 2023
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Multidrug-Resistant Bacteria Associated with Cell Phones of Healthcare Professionals in Selected Hospitals in Saudi Arabia.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2018
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

William L CodyDepartment of Microbiology, University of Colorado Denver, School of Medicine, Aurora, CO 80045, USA.
James W Wilson
David R Hendrixson
Kevin S McIver
Kayla E Hagman
C M Ott
Cheryl A Nickerson
Michael J Schurr

Funding

PRD-Containing Virulence Regulators of Pathogenic StreptococciR01AI047928 · NIAID · UT SOUTHWESTERN MEDICAL CENTER · PI MCIVER, KEVIN S. · 2001 to 2023
$6.5M
Decorin-binding proteins of Borrelia burgdorferiR01AI051332 · NIAID · UT SOUTHWESTERN MEDICAL CENTER · PI NORGARD, MICHAEL V · 2003 to 2007
$1.4M
Regulation of Ps. aeruginosa Virulence Factors by AlgRR01AI050812 · NIAID · UNIVERSITY OF COLORADO DENVER · PI SCHURR, MICHAEL JOHN · 2004 to 2008
$1.3M
NIAID NIH HHS R01 AI047928NIAID NIH HHS R01 AI050812NIAID NIH HHS R01 AI051332NIAID NIH HHS R01AI47928NIAID NIH HHS R01AI50812NIAID NIH HHS R01AI51332
6 · The paper itself

Abstract

The results from bacterial strain recovery efforts following hurricanes Katrina and Rita are reported. Over 90% of strains frozen in 10% skim milk were recovered whereas various recovery rates were observed for glycerol-stored stocks (56% and 94% of Escherichia coli, depending upon the laboratory). These observations led to a viability comparison of Streptococcus pyogenes, Campylobacter jejuni, Borrelia burgdorferi, Salmonella enterica subsp. Typhimurium, Pseudomonas aeruginosa and E. coli strains stored in glycerol or skim milk. In all bacteria examined, 10% skim milk resulted in significantly longer viability after thawing than 15% glycerol solutions currently used in most laboratories.

Indexed as

Microbial ViabilityMilkAnimalsBacteriaCryopreservationCryoprotective AgentsGlycerolCryoprotective AgentsGlycerol

Identifiers

PMID18573555
PMCPMC2551311

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.