ArticleDevelopmental biology2008
Impaired sperm fertilizing ability in mice lacking Cysteine-RIch Secretory Protein 1 (CRISP1).
Article in Developmental biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 162 citations in OpenAlex.
- Molecular interplay between sperm and oocyte: a narrative review.Human reproduction update · 2026Review
- Plasma proteomic signatures in HIV-infected individuals post-SARS-CoV-2 infection.BMC infectious diseases · 2025Article
- Glucose and pyruvate differentially modulate metabolic and redox dynamics during capacitation to enable fertilization competence in mouse sperm.Reproductive biology and endocrinology : RB&E · 2025Article
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- A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability.Scientific reports · 2024Article
- The rapidly evolving X-linkedeLife · 2024Article
- Distinct actions of testicular endocrine and lumicrine signaling on the proximal epididymal transcriptome.Reproductive biology and endocrinology : RB&E · 2024Article
- The Rapidly Evolving X-linkedbioRxiv : the preprint server for biology · 2024Article
- Expression of NELL2/NICOL-ROS1 lumicrine signaling-related molecules in the human male reproductive tract.Reproductive biology and endocrinology : RB&E · 2024Article
- Lumicrine signaling: Extracellular regulation of sperm maturation in the male reproductive tract lumen.Genes to cells : devoted to molecular & cellular mechanisms · 2023Review
- Where are all the egg genes?Frontiers in cell and developmental biology · 2023Review
- Potential Use of Tannin Extracts as Additives in Semen Destined for Cryopreservation: A Review.Animals : an open access journal from MDPI · 2022Review
- CEP128 is involved in spermatogenesis in humans and mice.Nature communications · 2022Article
- Plasmodium falciparum Cysteine Rich Secretory Protein uniquely localizes to one end of male gametes.Molecular and biochemical parasitology · 2022Article
- The Importance of Gene Duplication and Domain Repeat Expansion for the Function and Evolution of Fertilization Proteins.Frontiers in cell and developmental biology · 2022Review
- Arrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation.Journal of extracellular vesicles · 2021Article
- Flagellar energetics from high-resolution imaging of beating patterns in tethered mouse sperm.eLife · 2021Article
- Review
- A framework for high-resolution phenotyping of candidate male infertility mutants: from human to mouse.Human genetics · 2021Review
- Cysteine-Rich Secretory Proteins (CRISP) are Key Players in Mammalian Fertilization and Fertility.Frontiers in cell and developmental biology · 2021Review
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Mammalian fertilization is a complex multi-step process mediated by different molecules present on both gametes. Epididymal protein CRISP1, a member of the Cysteine-RIch Secretory Protein (CRISP) family, was identified by our laboratory and postulated to participate in both sperm-zona pellucida (ZP) interaction and gamete fusion by binding to egg-complementary sites. To elucidate the functional role of CRISP1 in vivo, we disrupted the Crisp1 gene and evaluated the effect on animal fertility and several sperm parameters. Male and female Crisp1(-/-) animals exhibited no differences in fertility compared to controls. Sperm motility and the ability to undergo a spontaneous or progesterone-induced acrosome reaction were neither affected in Crisp1(-/-) mice. However, the level of protein tyrosine phosphorylation during capacitation was clearly lower in mutant sperm than in controls. In vitro fertilization assays showed that Crisp1(-/-) sperm also exhibited a significantly reduced ability to penetrate both ZP-intact and ZP-free eggs. Moreover, when ZP-free eggs were simultaneously inseminated with Crisp1(+/+) and Crisp1(-/-) sperm in a competition assay, the mutant sperm exhibited a greater disadvantage in their fusion ability. Finally, the finding that the fusion ability of Crisp1(-/-) sperm was further inhibited by the presence of CRISP1 or CRISP2 during gamete co-incubation, supports that another CRISP cooperates with CRISP1 during fertilization and might compensate for its lack in the mutant mice. Together, these results indicate that CRISP proteins are players in the mammalian fertilization process. To our knowledge this is the first knockout mice generated for a CRISP protein. The information obtained might have important functional implications for other members of the widely distributed and evolutionarily conserved CRISP family.
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