ArticleCurrent biology : CB2008
Axoneme beta-tubulin sequence determines attachment of outer dynein arms.
Article in Current biology : CB, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Using the Culex pipiens sperm proteome to identify elements essential for mosquito reproduction.PloS one · 2023Article
- Proteomic Changes Associated With Sperm Fertilizing Ability in Meat-Type Roosters.Frontiers in cell and developmental biology · 2021Article
- The tubulin code and its role in controlling microtubule properties and functions.Nature reviews. Molecular cell biology · 2020Review
- Dissecting the role of the tubulin code in mitosis.Methods in cell biology · 2018Article
- Posttranslational Modifications of Tubulin and Cilia.Cold Spring Harbor perspectives in biology · 2017Review
- The tubulin code: molecular components, readout mechanisms, and functions.The Journal of cell biology · 2014Review
- Mutations in ZMYND10, a gene essential for proper axonemal assembly of inner and outer dynein arms in humans and flies, cause primary ciliary dyskinesia.American journal of human genetics · 2013Article
- Gene duplication, tissue-specific gene expression and sexual conflict in stalk-eyed flies (Diopsidae).Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Drosophila spermiogenesis: Big things come from little packages.Spermatogenesis · 2012Article
- Distinct functional roles of β-tubulin isotypes in microtubule arrays of Tetrahymena thermophila, a model single-celled organism.PloS one · 2012Article
- Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions.Nature reviews. Molecular cell biology · 2011Review
- Tubulin evolution in insects: gene duplication and subfunctionalization provide specialized isoforms in a functionally constrained gene family.BMC evolutionary biology · 2010Article
- Polyglutamylation: the GLU that makes microtubules sticky.Current biology : CB · 2010Article
- Article
- Cooperativity between the beta-tubulin carboxy tail and the body of the molecule is required for microtubule function.Cell motility and the cytoskeleton · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Axonemes of motile eukaryotic cilia and flagella have a conserved structure of nine doublet microtubules surrounding a central pair of microtubules. Outer and inner dynein arms on the doublets mediate axoneme motility [1]. Outer dynein arms (ODAs) attach to the doublets at specific interfaces [2-5]. However, the molecular contacts of ODA-associated proteins with tubulins of the doublet microtubules are not known. We report here that attachment of ODAs requires glycine 56 in the beta-tubulin internal variable region (IVR). We show that in Drosophila spermatogenesis, a single amino acid change at this position results in sperm axonemes markedly deficient in ODAs. Moreover, we found that axonemal beta-tubulins throughout the phylogeny have invariant glycine 56 and a strongly conserved IVR, whereas nonaxonemal beta-tubulins vary widely in IVR sequences. Our data reveal a deeply conserved physical requirement for assembly of the macromolecular architecture of the motile axoneme. Amino acid 56 projects into the microtubule lumen [6]. Imaging studies of axonemes indicate that several proteins may interact with the doublet-microtubule lumen [3, 4, 7, 8]. This region of beta-tubulin may determine the conformation necessary for correct attachment of ODAs, or there may be sequence-specific interaction between beta-tubulin and a protein involved in ODA attachment or stabilization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.