ArticleAnalytical biochemistry2008
Real-time PCR quantification using a variable reaction efficiency model.
Article in Analytical biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Article
- The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2022Article
- Saliva is Comparable to Nasopharyngeal Swabs for Molecular Detection of SARS-CoV-2.Microbiology spectrum · 2021Article
- Nucleic Acid Testing of SARS-CoV-2.International journal of molecular sciences · 2021Review
- A new method for quantitative real-time polymerase chain reaction data analysis.Journal of computational biology : a journal of computational molecular cell biology · 2013Article
- Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR.Nucleic acids research · 2012Article
- Article
- Selection of reference genes for qPCR in hairy root cultures of peanut.BMC research notes · 2011Article
- Experimental Validation of a Fundamental Model for PCR Efficiency.Chemical engineering science · 2011Article
- Interrogating the transgenic genome: development of an interspecies tiling array.Systems biology in reproductive medicine · 2011Article
- Efficiency of the Polymerase Chain Reaction.Chemical engineering science · 2010Article
- Article
- Silencing by nuclear matrix attachment distinguishes cell-type specificity: association with increased proliferation capacity.Nucleic acids research · 2009Article
- Differential nuclear scaffold/matrix attachment marks expressed genes.Human molecular genetics · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The quantitative real-time polymerase chain reaction (PCR) remains a cornerstone technique in gene expression analysis and sequence characterization. Despite the importance of the approach to experimental biology, the confident assignment of reaction efficiency to the early cycles of real-time PCR reactions remains problematic. Considerable noise may be generated when few cycles in the amplification are available to estimate peak efficiency. An alternate approach that uses data from beyond the log-linear amplification phase is explored in this article with the aim of reducing noise and adding confidence to efficiency estimates. PCR reaction efficiency is regressed to estimate the per-cycle profile of an asymptotically departed peak efficiency even when this is not closely approximated in the measurable cycles. The process can be repeated over replicates to develop a robust estimate of peak reaction efficiency. This leads to an estimate of the maximum reaction efficiency that may be considered primer design specific. Using a series of biological scenarios, we demonstrate that this approach can provide an accurate estimate of initial template concentration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.