Evidence map›Paper›PMID 18568348›Full record

ArticleCurrent genetics2008

Preparation of yeast mitochondrial DNA for direct sequence analysis.

Matus Valach, Lubomir Tomaska, Jozef Nosek

Abstract read
PubMed Publisher
In one paragraph

Article in Current genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Genome analysis of Candida subhashii reveals its hybrid nature and dual mitochondrial genome conformations.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Matus ValachDepartment of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynska dolina CH-1 and B-1, 842 15, Bratislava, Slovak Republic.
Lubomir Tomaska
Jozef Nosek
Comenius University Bratislava · SK

Funding

Studies of Telomere Structure Using Yeast Model SystemsR03TW005654 · FIC · UNIV OF NORTH CAROLINA CHAPEL HILL · PI GRIFFITH, JACK D · 2001 to 2008
$227k
FIC NIH HHS 2-R03-TW005654-04A1Howard Hughes Medical Institute 55005622
6 · The paper itself

Abstract

We describe two simple protocols for preparation of templates for direct sequencing of yeast mitochondrial DNA (mtDNA) by automatic DNA analyzers. The protocols work with a range of yeast species and yield a sufficient quantity and quality of the template DNA. In combination with primer-walking strategy, they can be used either as an alternative or a complementary approach to shot-gun sequencing of random fragment DNA libraries. We demonstrate that the templates are suitable for re-sequencing of the mtDNA for comparative analyses of intraspecific variability of yeast strains as well as for primary determination of the complete mitochondrial genome sequence.

Indexed as

CandidaDNA, MitochondrialGenome, FungalPolymorphism, GeneticSaccharomyces cerevisiaeSequence Analysis, DNATemplates, GeneticDNA, Mitochondrial

Identifiers

PMID18568348
OpenAlexW2005115865

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.