ArticleApplied microbiology and biotechnology2008
Regioselective synthesis of plant (iso)flavone glycosides in Escherichia coli.
Article in Applied microbiology and biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
- Biochanin A, a Plant Isoflavone, Disrupts Peptidoglycan Biosynthesis by DownregulatingAntibiotics (Basel, Switzerland) · 2026Article
- Review
- Similarities in Structure and Function of UDP-Glycosyltransferase Homologs from Human and Plants.International journal of molecular sciences · 2024Review
- Discovering New Substrates of a UDP-Glycosyltransferase with a High-Throughput Method.International journal of molecular sciences · 2024Article
- Biosynthesis and regulation of anthocyanin pathway genes.Applied microbiology and biotechnology · 2022Review
- Functional Characterization of a Novel Glycosyltransferase (UGT73CD1) from Iris tectorum Maxim. for the Substrate promiscuity.Molecular biotechnology · 2021Article
- De novo biosynthesis of bioactive isoflavonoids by engineered yeast cell factories.Nature communications · 2021Article
- Evolution-aided engineering of plant specialized metabolism.aBIOTECH · 2021Review
- A R2R3-MYB gene-based marker for the non-darkening seed coat trait in pinto and cranberry beans (Phaseolus vulgaris L.) derived from 'Wit-rood boontje'.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Metabolic Engineering of Microorganisms for the Production of Flavonoids.Frontiers in bioengineering and biotechnology · 2020Review
- Microbial Transformation of Flavonoids byMolecules (Basel, Switzerland) · 2019Article
- Probing the stereoselectivity of OleD-catalyzed glycosylation of cardiotonic steroids.RSC advances · 2018Article
- Glucuronidated Flavonoids in Neurological Protection: Structural Analysis and Approaches for Chemical and Biological Synthesis.Journal of agricultural and food chemistry · 2017Review
- Ameliorative effect of kaempferol, a flavonoid, on oxidative stress in streptozotocin-induced diabetic rats.Redox report : communications in free radical research · 2015Article
- When plants produce not enough or at all: metabolic engineering of flavonoids in microbial hosts.Frontiers in plant science · 2015Review
- Transcriptomic landscape of Pueraria lobata demonstrates potential for phytochemical study.Frontiers in plant science · 2015Article
- Understanding substrate selectivity of human UDP-glucuronosyltransferases through QSAR modeling and analysis of homologous enzymes.Xenobiotica; the fate of foreign compounds in biological systems · 2012Review
- Flavonoids: biosynthesis, biological functions, and biotechnological applications.Frontiers in plant science · 2012Article
- Article
- Structure, function, and engineering of enzymes in isoflavonoid biosynthesis.Functional & integrative genomics · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The flavonoids genistein, biochanin A, luteolin, quercetin, and kaempferol are plant natural products with potentially useful pharmacological and nutraceutical activities. These natural products usually exist in plants as glycosides, and their glycosylation has a remarkable influence on their pharmacokinetic properties. The glycosyltransferases UGT71G1 and UGT73C8 from Medicago truncatula are excellent reagents for the regioselective glycosylation of (iso)flavonoids in Escherichia coli grown in Terrific broth. Ten to 20 mg/L of either genistein or biochanin A 7-O-glucoside was produced after feeding genistein or biochanin A to E. coli expressing UGT71G1, and similar levels of luteolin 4'-O- and 7-O-glucosides were produced after feeding luteolin to cultures expressing UGT73C8. For the production of kaempferol 3-O-glucoside or quercetin 3-O-glucoside, the Phe148Val or Tyr202Ala mutants of UGT71G1 were employed. Ten to 16 mg/L of either kaempferol 3-O- or quercetin 3-O-glucosides were produced on feeding kaempferol or quercetin to E. coli expressing these enzymes. More than 90% of the glucoside products were released to the medium, facilitating their isolation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.