ArticleExtremophiles : life under extreme conditions2008
Evidence that the xylanase activity from Sulfolobus solfataricus Oalpha is encoded by the endoglucanase precursor gene (sso1354) and characterization of the associated cellulase activity.
Article in Extremophiles : life under extreme conditions, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Sulfolobales: Acidothermophilic archaea as models for biology and biotechnological applications.Engineering microbiology · 2026Review
- Structural and functional insights into extreme thermal stability and activity of two GH12 domains of a multidomain glycosidase from a hyperthermophilic euryarchaeon.The FEBS journal · 2025Article
- Extremophilic Microorganisms as a Source of Emerging Enzymes for the Food Industry: A Review.Food science & nutrition · 2025Review
- Archaea as a Model System for Molecular Biology and Biotechnology.Biomolecules · 2023Review
- Engineering ofJournal of microbiology and biotechnology · 2022Article
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Expression and characterisation of a thermophilic endo-1,4-β-glucanase from Sulfolobus shibatae of potential industrial application.Molecular biology reports · 2018Article
- Article
- Isolation and Characterization of the First Xylanolytic Hyperthermophilic Euryarchaeon Thermococcus sp. Strain 2319x1 and Its Unusual Multidomain Glycosidase.Frontiers in microbiology · 2016Article
- Multifunctional enzymes in archaea: promiscuity and moonlight.Extremophiles : life under extreme conditions · 2013Review
- Carbohydrate hydrolysis and transport in the extreme thermoacidophile Sulfolobus solfataricus.Applied and environmental microbiology · 2012Article
- Hyperthermophilic endoglucanase for in planta lignocellulose conversion.Biotechnology for biofuels · 2012Article
- Acidophilic bacteria and archaea: acid stable biocatalysts and their potential applications.Extremophiles : life under extreme conditions · 2012Review
- Genetic tool development underpins recent advances in thermophilic whole-cell biocatalysts.Microbial biotechnology · 2011Review
- A new archaeal beta-glycosidase from Sulfolobus solfataricus: seeding a novel retaining beta-glycan-specific glycoside hydrolase family along with the human non-lysosomal glucosylceramidase GBA2.The Journal of biological chemistry · 2010Article
- Thermostable enzymes as biocatalysts in the biofuel industry.Advances in applied microbiology · 2010Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sulfolobus solfataricus strain Oalpha was previously isolated for its ability to grow on minimal medium supplemented with xylan as a carbon source. The strain exhibited thermostable xylanase activity but several attempts to identify the gene encoding for the activity failed. Further studies showed that the xylanase displayed activity on carboxymethylcellulose (CMC) and the new activity was characterized. It exhibited an optimal temperature and pH of 95 degrees C and 3.5, respectively, and a half-life of 53 min at 95 degrees C. The enzyme, which was demonstrated to be glycosylated, hydrolyzed CMC in an endo-manner releasing cellobiose and other cello-oligomers. Analysis of the tryptic fragments by tandem mass spectrometry led to identification of the endoglucanase precursor, encoded by the sso1354 gene, as the protein possessing dual activity. The efficiency of the SSO1354 protein in degrading cellulosic and hemicellulosic fractions contained in agronomic residues was tested at low pH and high temperature. Cellulose and xylan were degraded to glucose and xylose at 90 degrees C, pH 4 by an enzyme mix consisting of SSO1354 and additional glycosyl hydrolases from S. solfataricus Oalpha. Given its role in saccharification processes requiring high temperatures and acidic environments, SSO1354 represents an interesting candidate for the utilization of agro-industrial waste for fuel production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.