ArticleNucleic acids research2008
Rapid and efficient construction of markerless deletions in the Escherichia coli genome.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 99 citations in OpenAlex.
- A recombineering-based platform for high-throughput genomic editing inApplied and environmental microbiology · 2025Article
- Engineering of a Substrate Affinity Reduced S-Adenosyl-methionine Synthetase as a Novel Biosensor for Growth-Coupling Selection of L-Methionine Overproducers.Applied biochemistry and biotechnology · 2024Article
- Exploring the Potential of a Genome-ReducedInternational journal of molecular sciences · 2023Article
- A MAD7-based genome editing system for Escherichia coli.Microbial biotechnology · 2023Article
- Application of Cloning-Free Genome Engineering toMicroorganisms · 2023Article
- Review
- High-Fidelity Cytosine Base Editing in a GC-Rich Corynebacterium glutamicum with Reduced DNA Off-Target Editing Effects.Microbiology spectrum · 2022Article
- Enhancing the Production of Pinene inBiology · 2022Article
- Potentials, prospects and applications of genome editing technologies in livestock production.Saudi journal of biological sciences · 2022Review
- Advances on systems metabolic engineering ofSynthetic and systems biotechnology · 2020Article
- ConvertingSynthetic and systems biotechnology · 2020Article
- Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics.Frontiers in microbiology · 2020Review
- Basal transcription profiles of the rhamnose-inducible promoter PCurrent genetics · 2019Article
- Antibiotic Combinations That Enable One-Step, Targeted Mutagenesis of Chromosomal Genes.ACS infectious diseases · 2018Article
- Coupling the recombineering to Cre-lox system enables simplified large-scale genome deletion in Lactobacillus casei.Microbial cell factories · 2018Article
- Restriction-deficient mutants and marker-less genomic modification for metabolic engineering of the solvent producerBiotechnology for biofuels · 2018Article
- A Multiplex Genome Editing Method forFrontiers in microbiology · 2018Article
- A Novel and Efficient Method for Bacteria Genome Editing Employing both CRISPR/Cas9 and an Antibiotic Resistance Cassette.Frontiers in microbiology · 2017Article
- Inducible Prophage Mutant ofFrontiers in microbiology · 2017Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process was mediated by a special plasmid, pREDI, which carries two independent inducible promoters: (i) an arabinose-inducible promoter that drives expression of lambda-Red recombination proteins, which carry out the replacement of a target genomic region with a marker-containing linear DNA cassette, and (ii) a rhamnose-inducible promoter that drives expression of I-SceI endonuclease, which stimulates deletion of the introduced marker by double-strand breakage-mediated intramolecular recombination. This genomic deletion was performed successively with only one plasmid, pREDI, simply by changing the carbon source in the bacterial growth medium from arabinose to rhamnose. The efficiencies of targeted region replacement and deletion of the inserted linear DNA cassette were nearly 70 and 100%, respectively. This rapid and efficient procedure can be adapted for use in generating a variety of genome modifications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.