ArticlePlant physiology2008
Imaging nutrient distributions in plant tissue using time-of-flight secondary ion mass spectrometry and scanning electron microscopy.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Unveiling organ-specific metabolism ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue.BMC biology · 2023Article
- Back to the basics of time-of-flight secondary ion mass spectrometry data analysis of bio-related samples. II. Data processing and display.Biointerphases · 2023Article
- Back to the basics of time-of-flight secondary ion mass spectrometry of bio-related samples. I. Instrumentation and data collection.Biointerphases · 2023Article
- The localization of the alkaloids inFrontiers in plant science · 2022Article
- Insight into chemical mechanisms of sepal color development and variation in hydrangea.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2021Review
- Localization of (+)-Catechin inMolecules (Basel, Switzerland) · 2020Article
- Salinity Effects on Sugar Homeostasis and Vascular Anatomy in the Stem of theInternational journal of molecular sciences · 2019Article
- Direct mapping of hydrangea blue-complex in sepal tissues of Hydrangea macrophylla.Scientific reports · 2019Article
- Lateral Transport of Organic and Inorganic Solutes.Plants (Basel, Switzerland) · 2019Review
- Biological tissue sample preparation for time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging.Nano convergence · 2018Review
- Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS.Scientific reports · 2017Article
- In Planta Localization of Stilbenes within Picea abies Phloem.Plant physiology · 2016Article
- Distribution of coniferin in freeze-fixed stem of Ginkgo biloba L. by cryo-TOF-SIMS/SEM.Scientific reports · 2016Article
- High-resolution secondary ion mass spectrometry reveals the contrasting subcellular distribution of arsenic and silicon in rice roots.Plant physiology · 2011Article
- Visualization of lateral water transport pathways in soybean by a time of flight-secondary ion mass spectrometry cryo-system.Journal of experimental botany · 2011Article
- Comparative physiology of elemental distributions in plants.Annals of botany · 2010Review
- Tracing cationic nutrients from xylem into stem tissue of French bean by stable isotope tracers and cryo-secondary ion mass spectrometry.Plant physiology · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new approach to trace the transport routes of macronutrients in plants at the level of cells and tissues and to measure their elemental distributions was developed for investigating the dynamics and structure-function relationships of transport processes. Stem samples from Phaseolus vulgaris were used as a test system. Shock freezing and cryo-preparation were combined in a cryogenic chain with cryo-time-of-flight secondary ion mass spectrometry (cryo-ToF-SIMS) for element and isotope-specific imaging. Cryo-scanning electron microscopy (cryo-SEM) was integrated into the cryogenic workflow to assess the quality of structural preservation. We evaluated the capability of these techniques to monitor transport pathways and processes in xylem and associated tissues using supplementary sodium (Na) and tracers for potassium (K), rubidium (Rb), and (41)K added to the transpiration stream. Cryo-ToF-SIMS imaging produced detailed mappings of water, K, calcium, magnesium, the K tracers, and Na without quantification. Lateral resolutions ranged from 10 microm in survey mappings and at high mass resolution to approximately 1 microm in high lateral resolution imaging in reduced areas and at lower mass resolution. The tracers Rb and (41)K, as well as Na, were imaged with high sensitivity in xylem vessels and surrounding tissues. The isotope signature of the stable isotope tracer was utilized for relative quantification of the (41)K tracer as a fraction of total K at the single pixel level. Cryo-SEM confirmed that tissue structures had been preserved with subcellular detail throughout all procedures. Overlays of cryo-ToF-SIMS images onto the corresponding SEM images allowed detailed correlation of nutrient images with subcellular structures.
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